Research Article Screening of Alkaline phosphatase activity in Bacteria

Authors

  • Hamid Ghaffoori Hasan Komar university of Science and technology, Pharmacy Dept, Kurdistan, IRAQ Author
  • Neerugatti Dora babu Komar university of Science and technology, Pharmacy Dept, Kurdistan, IRAQ Author

DOI:

https://doi.org/10.61841/asc5f240

Keywords:

ALP activity, Bacterium species, colorimetric Assay

Abstract

The study was conducted in the pathological analysis department during the period from 13th. NOV. To 30th. MAR 2017. Specimens used were different species of bacterium, by applying ALP activity determination in the broth of the bacterium species. The study reflects a higher total activity of ALP in Proteus species than the other species. While, specific activity was seen in E.Coli.

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References

1. Polgar, L., 1989. Mechanisms of Protease Action. CRC Press, Inc., Boca Raton, Florida, pp: 43-76.

2. Kim EE, Wyckoff HW (March 1991). "Reaction mechanism of alkaline phosphatase based on crystal structures. Two-metal ion catalysis". J. Mol. Biol. 218 (2): 449–64.

3. Tamás L, Huttová J, Mistrk I, Kogan G (2002). "Effect of Carboxymethyl Chitin-Glucan on the Activity of Some Hydrolytic Enzymes in Maize Plants" (PDF). Chem. Pap. 56 (5): 326–329.

4. Appendix E: Stem Cell Markers". Stem Cell Information. National Institutes of Health, U.S. Department of Health and Human Services. Retrieved 2013-09-24.

5. Stefková K, Procházková J, Pacherník J (2015). "Alkaline phosphatase in stem cells". STEM CELLS INTERNATIONAL. 2015: 628368.

6. Galdiero, Stefania; Falanga, Annarita; Cantisani, Marco; Tarallo, Rossella; Pepa, Maria Elena Della; D’Oriano, Virginia; Galdiero, Massimiliano (2017-03-08). "Microbe-Host Interactions: Structure and Role of Gram-Negative Bacterial Porins". Current Protein & Peptide Science. 13 (8): 843–854.

7. Horiuchi T, Horiuchi S, Mizuno D (May 1959). "A possible negative feedback phenomenon controlling formation of alkaline phosphomonoesterase in Escherichia coli". Nature. 183 (4674): 1529–30.

8. Coleman, Joseph E. (1992). "Structure and Mechanism of Alkaline Phosphatase". Annu. Rev. Biophys. Biomol. Struct. 21: 441–483.

9. Ammerman JW, Azam F (March 1985). "Bacterial 5-nucleotidase in aquatic ecosystems: a novel mechanism of phosphorus regeneration". Science. 227 (4692): 1338–40.

10. Wanner BL, Latterell P (October 1980). "Mutants affected in alkaline phosphatase, expression: evidence for multiple positive regulators of the phosphate regulon in Escherichia coli". Genetics. 96 (2): 353–66.

11. Garen A, Levinthal C (March 1960). "A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase". Biochim. Biophys. Acta. 38: 470–83.

12. Harada M, Udagawa N, Fukasawa K, Hiraoka BY, Mogi M (February 1986). "Inorganic pyrophosphatase activity of purified bovine pulp alkaline phosphatase at physiological pH". J. Dent. Res. 65 (2): 125–7.

13. Belfield A and Goldberg DM " Estimation of plasma phosphatases by determination of hydrolysed phenol with amino-antipyrine" 1971, 12:561-573

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Published

31.10.2020

How to Cite

Hasan, H. G., & babu, N. D. (2020). Research Article Screening of Alkaline phosphatase activity in Bacteria. International Journal of Psychosocial Rehabilitation, 24(8), 14575-14582. https://doi.org/10.61841/asc5f240