Laser Capture Micro-Dissection – A Premium Tool in Oral and Maxillofacial Pathology

Authors

  • Pallavi Mishra MDS, Senior Lecturer, Department of Oral and Maxillofacial Pathology, Kalinga Institute of Dental Sciences,KIIT Deemed to be University Bhubaneshwar, Odhisa, Author
  • Aishwariya Mohanty Post Graduate Trainee, Department of Oral and Maxillofacial Pathology, Kalinga Institute of Dental Sciences,KIIT Deemed to be University Bhubaneshwar, Odhisa, Author
  • Abikshyeet Panda Reader, Department of Oral and Maxillofacial Pathology, Kalinga Institute of Dental Sciences, KIIT Deemed to be University Bhubaneshwar, Odhisa, Author

DOI:

https://doi.org/10.61841/6wfn0z49

Keywords:

laser capture micro-dissection – a premium tool in oral and maxillofacial pathology

Abstract

The dawn of new technologies has enabled deeper insight into processes at subcellular levels, which ultimately improves diagnostic procedures and patient outcomes. Microdissection has greatly contributed to rapid growth in several areas, such as gene expression analysis, proteomics, genomics, and metabolomics. Laser capture microdissection (LCM) as a method of procuring subpopulations of cells under direct visual inspection plays a vital role in these areas. There is ample evidence to substantiate the utility of LCM in understanding the underlying molecular mechanism involving an array of oral physiological and pathological processes. This review provides the indispensable application of LCM in various oral disease from the perspective of an oral pathologist.

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References

1. Jayanandan M, Sujatha G, Patil S, Raj AT. Laser Capture Microdissection in Oral Cancer. J Contemp Dent Pr. 2018;19(5)::475-476.

2. Domazet B, Maclennan GT, Lopez-Beltran A, Montironi R, Cheng L. Laser capture microdissection in the genomic and proteomic era: targeting the genetic basis of cancer. Int J Clin Exp Pathol. 2008;1(6):475–88.

3. Suarez-Quian CA, Goldstein SR, Bonner RE. Laser capture microdissection: A new tool for the study of spermatogenesis. J Androl. 2000;21(5):601–8.

4. Emmert-Buck, M.R., Bonner, R.F., Smith, P.D., Chuaqui, R.F., Zhuang, Z., Goldstein, S.R., Weiss, R.A., Liotta LA. Laser capture microdissection. Science (80- ). 1996;274(5289):998-1001.

5. Virginia Espina, John Milia, Glendon Wu, Stacy Cowherd and LAL. Laser capture microdissection and quantitative-PCR analysis. Methods Mol Biol. 2006;319:213–29.

6. Virginia Espina, Michael Heiby MP and LAL. Laser capture microdissection. Mol Diagnostics Clin Lab. 2007;7(5):647–657.

7. Curran S, McKay JA, McLeod HL, Murray GI. Laser capture microscopy. J Clin Pathol - Mol Pathol. 2000;53(2):64–8.

8. karin Schutze GL. Identification of expressed genes by laser-mediated manipulation of single cells. Nat Biotechnol. 1998;16(8):737–42.

9. Maitra A, Gazdar AF. TISSUE MICRODISSECTION AND INTRODUCTION : METHODS FOR

OBTAINING PURE. Cancer Chemoprevention. 2001;106(9):63–84.

10. hennavan A, Sharma M, Chandrashekar C, Hunter K, Radhakrishnan R. Exploring the potential of laser capture microdissection technology in integrated oral biosciences. Oral Dis. 2017;23:737–48.

11. 11. Hunt JL, Finkelstein SD. Microdissection techniques for molecular testing in surgical pathology. Arch Pathol Lab Med. 2004;128(12):1372–8.

12. Fend F, V MR. Laser capture microdissection in pathology. J Clin Pathol. 2000;53(9):666–72.

13. Datta S, Malhotra L, Dickerson R, Chaffee S, Sen CK, Roy S. Laser capture microdissection: Big data from small samples. Histol Histopathol. 2015;30(11):1255–69.

14. Goldstein SR, Mcqueen PG, Bonner RF. Thermal modeling of Laser Capture Microdissection. Appl Opt. 1998;37(98):7378–91.

15. Standaert DG. Applications of Laser Capture Microdissection in the Study of Neurodegenerative Disease. ARCH NEUROL. 2005;62:203–5.

16. Wang X, Fan M, Chen X, Wang S, Jamal M, Wang L, et al. Intratumor genomic heterogeneity correlates with histological grade of advanced oral squamous cell carcinoma q. oral Oncol. 2006;42:740–4.

17. Dietmaier W, Hartmann A, Wallinger S, Heinmöller E, Kerner T, Endl E, et al. Multiple mutation analyses in single tumor cells with improved whole genome amplification. Am J Pathol. 1999;154(1):83–95.

18. Devilliers P, Suggs C, Simmons D, Murrah V, Wright JT. Microgenomics of ameloblastoma. J Dent Res. 2011;90(4):463–9.

19. 1Migaldi M, Pecorari M, Rossi G, Maiorana A, Bettelli S, Tamassia MG, et al. Does HPV play a role in the etiopathogenesis of ameloblastoma? An immunohistochemical, in situ hybridization and polymerase chain reaction study of 18 cases using laser capture microdissection. Mod Pathol. 2005;18(2):283–9.

20. Accurso BT, Warner BM, Knobloch TJ, Weghorst CM, Shumway BS, Allen CM, et al. Allelic imbalance in oral lichen planus and assessment of its classification as a premalignant condition. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011;112(3):359–66.

21. Chen W. Gene Expression Profile of Salivary Adenoid Cystic Carcinoma Associated with Perineural Invasion. Tohoku J Exp Med. 2007;212:319–34.

22. Maehara T, Moriyama M, Hayashida J, Tanaka A, Nakamura S. Selective localization of T helper subsets in labial salivary glands from primary Sjögren ’ s syndrome patients. Clin Exp Immunol. 2012;169:89–99.

23. imone NL, Paweletz CP, Charboneau LU, Iii EFP, Liotta LA. Laser Capture Microdissection : Beyond Functional Genomics to Proteomics. Mol diagnosis. 2000;5(4):301–7.

24. Patel V, Hood BL, Molinolo AA, Lee NH, Conrads TP, Braisted JC, et al. Human Cancer Biology Proteomic Analysis of Laser-Captured Paraffin-Embedded Tissues : A Molecular Portrait of Head and Neck Cancer Progression. 2008;14(8):1002–15.

25. Yuan Y, Zhou Y, Zhang D, Zeng X. Screening diagnostic biomarkers of OSCC via an LCM-based proteomic approach. Oncol Rep. 2018;40:2088–96.

26. Aoki T, Tsukinoki K, Karakida K, Ota Y, Otsuru M, Kaneko A. Expression of cyclooxygenase-2, Bcl-2 and Ki-67 in pleomorphic adenoma with special reference to tumor proliferation and apoptosis. Oral Oncol. 2004;40(9):954–9.

27. He H, Sun G, Ping F. Laser-capture microdissection and protein extraction for protein fingerprint of OSCC and OLK. Artif Cells, Blood Substitutes, Biotechnol. 2009;37(5):208–13.

28. Li M, Firth JD, Putnins EE. Keratinocyte growth factor-1 expression in healthy and diseased human periodontal tissues. J Periodontal Res. 2005;40(2):118–28

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Published

31.10.2020

Issue

Vol. 24 No. 8 (2020): 24 (8) 2020

Section

Articles

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How to Cite

Mishra, P., Mohanty, A., & Panda, A. (2020). Laser Capture Micro-Dissection – A Premium Tool in Oral and Maxillofacial Pathology. International Journal of Psychosocial Rehabilitation, 24(8), 14314-14321. https://doi.org/10.61841/6wfn0z49