Minimum inhibition concentration of two antibiotics and 1 two plants extract against Mycoplasma galisepticum in 2 Iraq
DOI:
https://doi.org/10.61841/qs0qgj96Keywords:
Mycoplasma galisepticum, MIC, antibiotics, extract, IraqAbstract
This study was designed in order to evaluate the effectives of two antibiotics and two 9
plant extracts against Mycoplasma galisepticum. Two commercial antibiotics 10
tilmicosin and enrofloxacin used for minimum inhibition concentration detection 11
against Mycoplasma galisepticum isolates. Two plants extracts also used for the same 12
purpose which is garlic and thyme. Using the micro-broth technique to determine the 13
growth of in vitro activities of tilmicosin, enrofloxacin, garlic and thyme against the 14
isolates of Mycoplasma gallisepticum. The results showed that the tilmicosin had 15
MICs of 7.8 mg/ml. while, enrofloxacin showed the best in vitro activity giving MICs 16
of 1.56 mg/ml. Garlic had MICs of 0.97 mg/ml which was the best of all results. 17
Lastly thyme had MICs of 15.6 mg/ml for the Mycoplasma gallisepticum. According 18
to the results all Mycoplasma gallisepticum strains are sensitive to all antibiotics and 19
plants extracts in different level and the highest effectiveness was for the garlic.
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References
Abo El-Ela, F.I. and El-Banna, H.A. (2017). Exploring novel medical applications for 277
commonly used veterinary drug (tilmicosin antibiotic). Insights in Veterinary 278
Science, 1: 1-16. https://doi.org/10.29328/journal.ivs.1001001. 279
Abu-Basha, N.; Idkaidek, A. and Al-Shunnaq, A. (2007). Pharmacokinetics of 280
Tilmicosin (Provitil Powder and Pulmotil Liquid AC) Oral Formulations in 281
Chickens.Veterinary Research Communications, 31 (4): 477–485. 282
Afifi, F.U. and Abu-Irmaileh, B. (2000). Herbal medicine in Jordan with special 283
emphasis on less commonly used medicinal herbs. Journal of 284
Ethnopharmacology, 72: 101–111. 285
Ahmad, A.; Davies, J.; Randall, S. and Skinner, G.R.B. (1996). Antiviral properties of 286
extract of Opuntia streptacantha. Antiviral Research, 30: 75–85. 287
Ali, A.J. (2019). Isolation, identification and some aspects pathogenicity of 288
Mycoplasma gallisepticum in broiler chickens. Ph.D. Thesis, College of 289
Veterinary Medicine, University of Baghdad. 290
Almanama, M.A. (2011). Prevalence of Mycoplasma gallisepticum in the Ten 291
licensed Hatcheries in Gaza strip, Palestine. A Master of Biological Sciences 292
Thesis / Medical Technology.
Al-Maqtari, M.A.A.; Alghalibi, S.M. and Alhamzy, E.H. (2011). Chemical 294
composition and antimicrobial activity of essential oil of Thymus vulgaris from 295
Yemen. Turk J Biochem, 36: 342-349. 296
Al-Momani, W.; Abu-Basha, E.; Janakat, S.; Nicholas, R.A.J. and Ayling, R.D. 297
(2007). In vitro antimycoplasmal activity of six Jordanian medicinal plants 298
against three Mycoplasma species. Trop Anim Health Prod, 39: 515–519. 299
Bajpai, M.; Pande, A.; Tewari, S.K. and Prakash, D. (2005). Phenolic contents and 300
antioxidant activity of some food and medicinal plants. Int. J. Food Sci. Nutr., 301
56 (4): 287-291. 302
Committee for Medicinal Products for Veterinary Use (CVMP) (2007). Public 303
Statement on the Use of (Fluoro) Quinolones in Food-Producing Animals in the 304
European Union: Development of Resistance and Impact on Human and Animal 305
Health. 306
Eisenberg, D.M.; Kessler, R.C.; Foster, C.; Norlock, F.E.; Calkins, D.R. and 307
Delbanco, T.L. (1993). Unconventional medicine in the United States: 308
prevalence, costs and patterns of use. New England Journal of Medicine, 328: 309
246–252. 310
Fauchier, N. (2013). Med’Vet, le recueil des spécialités à usage vétérinaire. 2014th 311
Edition, Med’com, Paris. 312
Friesen, N.; Fritsch, R.M. and Blattner, F.R. (2006). Phylogeny and new intrageneric 313
classification of Allium L. (Alliaceae) based on nuclear ribosomal DNA its 314
sequences. Aliso., 22: 372-395. 315
Gama, H. (2008). Drug Utilization Studies. Arq Med., 22: 69-74. Ref.: 316
Gheith, I.; El-Mahmoudy, A.; Elmajdoub, A. and Awidat, A. (2015). 318
Pharmacovigilance of Tilmicosin in Mice. Act Scient Vet., 43: 1318. Ref.: 319
Grodio, J.; Buckles, E. and Schat, K. (2009). Production of house finch (Carpodacus 321
mexicanus) IgA specific anti-sera and its application in immunohistochemistry 322
and in ELISA for detection of Mycoplasma gallisepticum-specific IgA Vet. 323
Immun. and Immunopathol. J., 132: 288-294. 324
Grohe, K.; Zeiler, H.J.; Metzger, K.G. and Grohe, K. (1987). 7-Amino-1- 325
Cyclopropyl-4-Oxo-1, 4-Dihydro-Quinoline and Naphthyridine-3-Carboxylic 326
Acids and Antibacterial Agents Containing These Compounds. US4670444 (A)
http://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=198706 328
02&DB=EPODOC&locale=en_EP&CC=US&NR=4670444A&KC=A&ND=5. 329
Jaber, M.A. and Al-Mossawi, A. (2007). Susceptibility of some multiple resistant 330
bacteria to garlic extracts. Afr. J. Biotechnol., 6 (6): 771-776. 331
Jameel, M.M. and Hasso, S.M. (2018). Molecular detection of Mycoplasma 332
agalactiae and Mycoplasma capricolum in mastitic and non mastitic milk of 333
goats by using Real Time Polymerase Chain Reaction. The Iraqi Journal of 334
Veterinary Medicine, 42(1): 1-6. 335
Josling, P.A. (2005). The heart of garlic Nature's aid to healing the human body, HEC 336
Publishing, Chicago Illinois. Pp: 20. 337
Kempf, I.; Reeve, L.; Gesbert, F. and Guittet, M. (1997). Efficacy of tilmicosin in the 338
control of mycoplasma gallisepticum infection in chickens. Avian Dis., 41(4): 339
802–807. 340
Lanzotti, V. (2006). The analysis of onion and garlic. J. Chromat. A., 12 (1): 3-22. 341
Levisohn, S. and Kleven, S.H. (2000). Avian mycoplasmosis (Mycoplasma 342
gallisepticum). In: Diseases of poultry: world trade and public health 343
implications (C.W., Beard and M.S., McNulty; eds.), Rev. Sci. Tech. off Int. 344
Epiz., 19(2): 425-442. 345
Ley, D.H. (2003). Mycoplasma gallisepticum infection. In: Saif, Y.M.; Barnes, H.J.; 346
Fadly, A.M.; Glisson, J.R.; McDougald, L.R.; Swayne, D.E.; Diseases of 347
Poultry. Ames: Iowa State University Press. pp: 722-727. 348
Marjorie, M.C. (1999). Plant products as antimicrobial agents. Clinical Microbiology 349
Reviews, 12: 564–582. 350
Moerman, D.E. (1996). An analysis of the food plants and drug plants of native North 351
America. Journal of Ethnopharmacology, 52: 1–22. 352
Mossad, E.; Ashraf, E.; Mohamed, A. and Mohamed, M. (2014).Tissue Residues, 353
Hematological and Biochemical Effects of Tilmicosin in Broiler Chicken. 354
Veterinary Medicine International, Article ID 502872, 6 pages. 355
Nascimento, E.R.; Pereira, V.L.A.; Nascimento, M.G.F. and Barreto, M.L. (2005). 356
Avian mycoplasmosis update. Brazilian Journal of Poultry Science. 7(1): 1-9. 357
Nisha, A. (2008). Antibiotic residues - a global health hazard. Vet. World J., 12: 375- 358
377
Onyeagba, R.; Ugbogu, O.C.; Okeke, C.U. and Iroakasi, O. (2004). Studies on the 360
antimicrobial effects of garlic (Allium sativum L.), ginger (Zingiber officinale 361
Roscoe) and lime (Citrus aurantifolia L.). Afr. J. Biotechnol., 3: 552-554. 362
Oran, S. and Al-Eisawi, A. (1998). Check list of medicinal plants in Jordan. Dirasat, 363
25: 84–112. 364
Papich, M.G.; Riviere, J.E. and Ames, A. (2001). Chloramphenicol andderiva-tives, 365
macrolides, lincosamides, and miscellaneous antimicro-bials. In: Veterinary 366
Pharmacology and Therapeutics, Adams, H.R.E.; ed. (Iowa, USA, Iowa State 367
University Press), pp: 868-897. 368
Parekh, J. and Chanda, S. (2007). In vitro antimicrobial activity of Trapa natans L. 369
Fruit rind extracted in different solvents. Afr. J. Biotechnol., 6 (6): 766-770. 370
Peebles, E.D.; Branton, S.L. (2012). Mycoplasma gallisepticum in the commercial 371
egg-laying hen: an historical perspective considering effects of pathogen strain, 372
age of bird at inoculation, and diet on performance and physiology. The Journal 373
of Applied Poultry Research, 21(4): 897–914. 374
Reddy, P.; Kandisa, R.V.; Varsha, P.V. and Satyam, S. (2014). Review on Thymus 375
vulgaris Traditional Uses and Pharmacological Properties. Medicinal and 376
Aromatic Plants, 3 (3): doi: 10.4172/2167-0412.1000164. 377
Sarkozy, G. (2001). Quinolones: A Class of Antimicrobial Agents. Veterinární 378
Medicína (Praha), 46: 257-274. 379
Stockwell, C. (1988). Nature’s pharmacy. Century Hutchinson Ltd., London, United 380
Kingdom. 381
Sutardi, L.N.; Wientarsih, I.; Handharyani, E.; Andriani, and Agus Setiyono, A. 382
(2015). Indonesian Wild Ginger (Zingiber sp) Extract: Antibacterial Activity 383
against Mycoplasma gallisepticum. IOSR Journal Of Pharmacy, 5 (10): 59-64. 384
Taylor-Robinsona, D. and Bebearb, C. (1997). Antibiotic susceptibilities of 385
mycoplasmas and treatment of mycoplasmal infections. Journal of 386
Antimicrobial Chemotherapy, 40: 622–630. 387
Tepe, B.; Daferera, D.; Sokmen, M.; Polissiou, M. and Sokmen, A. (2004). In vitro 388
antimicrobial and antioxidant activities of the essential oils and various extracts 389
of thymus. J. Agric. Food Chem., 52: 1132-1137. 390
Timbo, B.B.; Ross, M.P.; McCarthy, P.V. and Lin, C.T. (2006). Dietary supplements 391
in a national survey: Prevalence of use and reports of adverse events. Am. Diet 392
Assoc., 106 (12): 1966-1974.
Trouchon, T. and Lefebvre, S. (2016). A Review of Enrofloxacin for Veterinary Use. 394
Open Journal of Veterinary Medicine, 6: 40-58. 395
Tsao, S.M. and Yin, M.C. (2001). In vitro antimicrobial activity of four diallyl 396
sulphides occurring naturally in garlic and Chinese leek oil. J. Med. Microbiol., 397
50: 646-649. 398
Wojdylo, A.; Oszmianski, J. and Czemerys, R. (2007). Antioxidant activity and 399
phenolic compounds in 32 selected herbs. Food Chem., 105: 940-949. 400
Wright, D.H.; Brown, G.H.; Peterson, M.L. and Rotschafer, J.C. (2000). Application 401
of Fluoroquinolone Pharmacodynamics. Journal of Antimicrobial 402
Chemotherapy, 46: 669-683. http://dx.doi.org/10.1093/jac/46.5.669. 40
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