The Potential Aqueous Extracts of Medical Plant and Alum against Bacteria, an in Vitro Study

Authors

  • Dr. Lubna Abdul Muttalib Al Shalah environmental research center, Babylon Province, Iraq Author

DOI:

https://doi.org/10.61841/02en7d03

Keywords:

Value

Abstract

Background: The attention for plants as well as to medicinal herbs has increased. It’s mainly as key sources for the foundation of medical drugs and a source of effective materials that go into the composition of the drug. Aim: In order to determine the medical significance of (Malvasylvestris, Matricariachmomilla, Salvia officinalis, Quercus infectoria, Curcuma Longa, and Alum)aqueous extract and its anti-microbial activity in contradiction of particular kinds within Gram positive, negative bacteria through incomes degree the distance of the inhibition zone against some types of Gram positive and negative bacteria by means measure the diameter of the inhibition zone (mm) Methods: These bacteria were randomly isolated from clinical samples of people whom Fallen asleep in Al-Hilla teaching hospital, Babylon during a period from October2016 to January 2017. In the present study; the diagnosis of bacterial strains where after its culturing proceeding within appropriate media (morphological feature) and then the diagnosis was performed by using conventional biochemical tests and Epi 20 system at the microbiological laboratory/College of Medicine/Babylon University. These strains were included; E.coli, Enterobacter, Klebsiella, Acinetobacter, proteus, Salmonella, and Pseudomonas. The results of this investigation have exposed that studied extract is effective in discouraging the development of these bacteria outside the body of the organism (Gram positive & Negative bacteria). CONCLUSION: from the above experiment, it was concluded that studied aqueous plant extracts has beneficial effect on tested pathogenic bacteria due to high concentration of energetic biochemical ingredient for antimicrobial action that useful for preventing certain enteric diseases.

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References

[1] Abeysinghe PD. Antibacterial Activity of some Medicinal Mangroves against Antibiotic Resistant Pathogenic Bacteria. Indian J Pharm Sci 2010; 72(2): 167-172.

[2] Pirbalouti AG, Yousefi M, Heshmetollah N, Karimi I, Koohpayeh A. Evaluation of burn healing properties of Arnehiaeuchroma and Malvasylvestris. Electron J Biol. 2009; 5: 62–66.

[3] Ozkan AM, Kutselm O. Anti-ulcerogenic activity of some plants used in folk medicine of pinnarbasi. J Ethnopharmacol. 2005; 101: 313–318

[4] Shivananda B, Sivachandra RS, Rao AV. Wound healing activity of matricariarecutita L. extract. J Wound Care. 2007; 16(7).

[5] Alkan F, Gursel F, Ates A. Protective effect of Salvia officinalis extract against cyclophosphamide induced genotoxicity and oxidative stress in rats. Turk J Vet Anim Sci. 2012; 36(6): 646-654.

[6] Jamzad Z, Panahi P, Mohammad RP, Fallha AM. Foliar epidermal morphology in Quercus (subgenus Quercus, section Quercus) in Iran. Acta Bot Croatia. 2012;71:95–13

[7] S. Fatima, A.H.A Farooqi, R. Kumar, T.R.S. Kumar, S.PS. Khanuja, “Antibacterial activity possessed by medicinal plants used in tooth powders,” J. Med Aromatic Plant Sci 2001; 22: 187-9.

[8] M. Digraki, M.H. Alma, A. Ilcim, and S. Sen, “Antibacterial and antifungal effects of various commercial plant extracts,” Pharm Biol., vol.37, pp. 216-20, 1993 [3] A.

[9] Redwane, H.B. Lazrek, S. Bouallam, M. Markouk, H. Amarouch, and M. Jana, “Larvicidal activity of extracts from Quercuslusitania var. infectoria galls (Oliv.),” J. Ethnopharmacol, vol. 79, pp. 261-3, 2002.

[10] J.K. Hwang, T.W. Kong, TW, N.I. Baek, and Y.R. Pyun, “α-Glycosidase Inhibitory Activity of hexagalloyl glucose from the galls of Quercusinfectoria,” Planta Med, vol. 66, pp. 73-4, 2000.

[11] M.R.Dar,S, Ikram, M, Fakouhi T, “Pharmacology of Quercusinfectoria”, J. Pharm Sci, vol.65, pp.1791-4, 1976

[12] G. Hussein, H. Miyashiro, N. Nakamura, M. Hattori, N. Kakiuchi, and K. Shimotohno, “Inhibitory effects of Sudanese medicinal plant extracts on hepatitis C virus protease,” Phytother Res., vol.14, pp.510-6, 2000.

[13] G. Kaur, H. Hamid, A. Ali, MS Alam, and M. Athar, “Antiinflammatory evaluation of alcoholic extract of galls of Quercusinfectoria,” J.Ethnopharmacol. vol.90, pp 285-92, 2004.

[14] Awasthi PK, Dixit SC. Chemical composition of Curcuma Longa leaves and rhizome oil from the plains of Northern India. J Young Pharm. 2009; 1: 312–6.

[15] Roses IA. Medicinal plants of the World: chemical Constituents, Traditional and Modern Medicinal Uses. New Jersey: Human Press; 1999. pp. 139–53.

[16] Zachariah TJ, Baby KN. Effect of storage of fresh turmeric rhizomes on oleoresion and curcumin contents.

J Spices Arom Crops. 1992; 1: 55–8.

[17] Srimal RC. Turmeric-a brief review of medicinal properties. Fitoterapia. 1997; 68: 483–93.

[18] Aggarwal BB, Harikumar KB. Potential therapeutic effects of curcumin, the antiinflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases. Int J Biochem Cell Biol. 2009; 41: 40–59.

[19] Bestoon, M.F. 2012. Evidance for feasibility of aluminum potassium sulfate (alum) solution as a root canal irrigant. J. Bagh. Coll. Dent. 24, 1-5.

[20] Awwad, A.M., Albiss, B.A, Salem N.M. Antibacterial Activity of synthesized Copper Oxide Nanoparticles using Malvasylvestris Leaf Extract. SMU Medical Journal, 2015; 2 (1):91-101.

[21] Courvalin, P.H. Jacquier, P. Cruaud, I. Poilane, E. Lachassinne, J. Gaudelus, A. Collignon, Cinétique de l' activitébactéricide. In: Antibiogramme Paris: ESKA 2006; 635.

[22] Barret, JP; Ramzy, PI; Heggers, JP; Villareal, C; Herndon, DN; Desai, MH. (1999). Topical nystatin powder in severe burns : a new treatment for angioinvasive fungal infections refractory to other topical and systemic agents.; 25(6):505-8

[23] Nabi Shariatifar1, Ayub Ebadi Fathabad, Gholamreza Jahed Khaniki1 and Hassan Gandomi. Evaluation of the antibacterial activity of essential oil and aqueous and ethanolic extracts of Quercusinfectoria leaves on food-borne pathogenic bacteria. International Journal of Pharma Sciences and Research (IJPSR). 2014. ISSN: 0975-9492 (5)709-713.

[24] Harison Masih, Jyotsna Kiran Peter1 and Pratima Tripathi. A comparative evaluation of antifungal activity of medicinal plant extracts and chemical fungicides against four plant pathogens. Int. J. Curr. Microbiol. App. Sci (2014) 3(5): 97-109.

[25] IlhamAbassBnyan, Abdulsamie Hassan Alta'ee, Nadia Hassan Kadhum. Antibacterial Activity of Aluminum Potassium Sulfate and Syzygium Aromaticum Extract Against Pathogenic Microorganisms. Journal of Natural Sciences Research 2014; ISSN 2225-0921(4)15:11-15.

[26] Nada Khazal Kadhim Hindi. Husein Abbas Rehim Alsultany, Ifad Kerim Abd Alshibly, Zainab Adil Ghani Chabuck. The effect of some medical plants on bacterial skin infections. World Journal of Pharmacy and Pharmaceutical Sciences 2013.2, 5, 2355-2366.

[27] Muhammad H, Muhammad S (2005). The used of Lawsoniainermis Linn. (Henna) in the management of burn wound infections. Afr. J.Biotechnol., 4(9): 934-937.

[28] Todar, K. 2007. Pathogenic E. coli. Online Textbook of Bacteriology. University of Wisconsin, Madison. Department of Bacteriology.

[29] De Souza GC, Haas AP, von Poser GL, Schapoval EE, Elisabetsky E. Ethnopharmacological studies of antimicrobial remedies in the south of Brazil. J Ethnopharmacol 2004; 90(1):135-43.

[30] Cheng CL, Wang ZY. Bacteriostasic activity of anthocyanin of Malvasylvestris. J For Res 2006; 17(1):83- 5.

[31] Yeole, N. B.; Sandhya, P. Chaudhari, P. S. and Bujbal, P. S. (2010). Evaluation of Malvasylvestris and Pedalium murex mucilage as suspending agent. Internat. J. Pharm Tech. Res., 2(1): 385-389.

[32] Serafini M, Peluso I, Raguzzini A. Flavonoids as anti-inflammatory agents. Proc Nutr Soc 2010; 69: 273e8.

[33] Dimidi E, Christodoulides S, Fragkos KC, Scott SM, Whelan K. The effect ofprobiotics on functional constipation in adults: a systematic review and metaanalysisof randomized controlled trials. Am J Clin Nutr 2014; 100: 1075e84.

[34] Bouzouita, N.; Kacouri, F. Homdi, M. And chabbouni, M. "Antimicrobial activity of essential oils from Tunisian arommatic plants" Flavour. Frag. J. 2003.18(15) : 639-649

[35] P. Feeny, Inhibitory effect of Oak Leaf Tannins on the Hydrolysis of proteins by Trypsine, J. Phytochemistry, 8, 2116, (1998)

[36] R.L. Lindroth and M.S. Bloomer, Biochemical ecology of foresttent caterpillar response to dietry protein and phenolic glycosides, J. Env. Emtocol., 86, 408 – 413, (1991) .

[37] Oh J, Heonjoo J, Ah RC, Sung-Jin K, Jaejoon H, Antioxidant and antimicrobial activities of various leafy herbal teas, Food Control. Vol. 31, No. 2, 2013, pp. 403-409.

[38] Wahba NM, Amany SA, Zedan ZE, Antimicrobial Effects of Pepper, Parsley, and Dill and Their Roles in the Microbiological Quality Enhancement of Traditional Egyptian Kareish Cheese. Foodborne Pathogens and Disease, Vol. 7, No. 4, 2010, pp. 411-418.

[39] P. Capek and V. Hribalova, Water-Soluble Polysaccharides from Salvia Officinalis L. Possessing Immunomodulatory Activity, Phytochem., 65, 1983-1992 (2004).

[40] Dar, M. S., Ikram, M. and Fakouhi, T. 1976. Pharmacology of QuercusInfectoria. Journal of Pharmaceutical Sciences 65: 1791-4

[41] Ikram, M. and Nowshad, F. 1977. Constituent of QuercusInfectoria. Plant Medicine 31: 286-7.

[42] Leela, T. and Satirapipathkul, C.2004. Studies on the Antibacterial Activity of Quercus Infectoria Galls. Proceedings of the International Conference on Bioscience, Biochemistry and Bioinformatics, March 10- 11, Singapore: IPCBEE, 5.

[43] Chandrana H, Baluja S, Chanda SV. Comparison of antibacterial activities of selected species of Zingiberaceae family and some synthetic compounds. Turk J Biol. 2005; 29(29):83–97

[44] Kim JH, Gupta SC, Park B, Yadav VR, Aggarwal BB. Turmeric (Curcuma longa) inhibits inflammatory nuclear factor (NF)-κB and NF-κB-regulated gene products and induces death receptors leading to suppressed proliferation, induced chemosensitization, and suppressed osteoclastogenesis

[45] Ammon, H.P.T. and M.A. Wahl. 1991. Pharmacology of Curcuma longa. Planta Med., 57: 1-7.

[46] Cikrikci, S.; Mozioglu, E. and Yilmaz, H. (2008) Biological activity of curuminoids isolated from Curcuma

longa. Rec. Nat. Prod., 2(1):19-24.

[47] Pundir, R.K. and Jain, P. (2010) Comparative studies on the antimicrobial activity of black pepper (Piper nigrum) and turmeric (Curcuma longa) extracts. Int. J. Appl. Biol. Pharmac. Technol., 1(2):492-501.

[48] Aggarwal, B.B., Sundaram, C., Malani, N. and Ichikawa, H. (2007) "Curcumin: the Indian solid gold". Adv. Exp. Med. Biol., 595:1-75.

[49] Singh, R.P. and Jain, D.A. (2011) Evaluation of antimicrobial activity of volatile oil and total curcuminoids extracted from turmeric. Int. J. of ChemTech Res., 3(3):1172-1178.

[50] Cikrikci, S.; Mozioglu, E. and Yilmaz, H. (2008) Biological activity of curuminoids isolated from Curcuma longa. Rec. Nat. Prod., 2(1):19-24

[51] Funk, J.L., Frye, J.B., Oyarzo, J.N., Zhang, H. and Timmermann, B. N. (2010) Anti-Arthritic Effects and Toxicity of the Essential Oils of Turmeric (Curcuma longa L.). J. Agric. Food Chem., 58(2):842-849.

[52] Rasooli, I. (2007) Food preservation-a biopreservative approach. Food, 1(2):111-136.

[53] Mallinckreod chemical. J. Bakr. Inc. 2009. Material and safety Data Sheet (MSDS). Aluminum potassiumsulfate. USA.

[54] Teixeira CS, Felippe MCS, Felipp WT. The effect of application time of EDTA and Naocl on intra canal smear layer removal: an SEM analysis. IntEndod J 2005; 38: 285-90

[55] Ahmed Saleem Abbas; Jeberson W, Klinsega V.V, “A Literature Review and Classification of Selected Software Engineering Researches”, International Journal of Engineering and Technology. 2012, 2(7): 1256-1282.

[56] Nada Khazal Kadhim Hindi. Bacteriological Study of Pigmented Bacteria and Molecular Study Based for 16s rRNA Gene. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2016, 7(6): 258- 266.

[57] Nada Khazal Kadhim Hindi. In vitro Antibacterial activity of Plants extracts against Porphyromonas gingivalis, Prevotella intermedia and Aggregatibacter actinomycetemcomitans Streptococcus mutanus, Isolated from Periodontitis Patients in Babylon province, Iraq. International Journal of PharmTech Research, 2016, 9 (11):84-93

[58] Nada Khazal Kadhim Hindi, Iman Fadhil Abdul-Husin, Mays Hadi Jebur, Zainab Khudhur Ahmad Al- Mahdi, Ammar Khazaal Kadhim. Evaluation of anti-bacterial activity of the aquatic henna leaves extract in Hilla City, an in vitro study, Journal of Chemical and Pharmaceutical Sciences, Iraq, Journal of Chemical and Pharmaceutical Sciences. 2017, 10 (1): 162-165.

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Published

30.06.2020

How to Cite

Shalah, D. L. A. M. A. (2020). The Potential Aqueous Extracts of Medical Plant and Alum against Bacteria, an in Vitro Study. International Journal of Psychosocial Rehabilitation, 24(4), 3945-3954. https://doi.org/10.61841/02en7d03