Phagocytic Activity of Neutrophils in the Blood of Pigs that Received A “Microecological System” Alternative to Antibiotics
DOI:
https://doi.org/10.61841/h3fcp877Keywords:
Blood, Neutrophils, Lactobacillus Plantarum, Antibiotic ReplacementAbstract
The study was aimed at the identification of a certain link of the nonspecific protection in pigs that enhances its activity during the application of different feeding plans with the probiotic "EM-Vita," creates a stable “microecological system," and provides high natural resistance. Weaned piglets aged 46-53 days were distributed into 3 groups by the method of analog pairs: Group I—the control group; Group 2—and Group 3—the test groups that received even doses of the drug but at different intervals. It was established that in 2 months, pigs that received “EM-Vita” by both schemes had expressed an increase in the level of nonspecific protection due to a general increase in the neutrophil count and their fractions capable of both oxygen-dependent microbicide activity and enhanced activity of this process. The application of the drug by one of the schemes provided the creation of the adaptive reserve of neutrophils that exert adsorptive activity by the other scheme—the increase in the synthesis of cationic proteins. Thus, the periodicity of the application of the probiotic “EM-Vita” significantly influences the status of neutrophils by selective activation of different protective systems.
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References
[1] Babayan, V.A., Kolomytsev, A.A., Gevorkyan, A.S. (1988). Resection of spontaneous rosette formation of
B-lymphocytes in pigs with mice erythrocytes. Veterinary, 11, 35-39.
[2] Betin, A.N. (2016). Effeciency of probiotic “BioPlus” YC"—plus 5 piglets. Pig breeding. 2016. № 5. p. 36-
40.
[3] Chumachenko, V.E., Vysotskiy, A.M., Serdyuk, N.A., Chumachenko, V.V. (1990). Evaluation of natural
resistance and metabolism in agricultural animals. Kiev: Urozhai.
[4] Collado, M.C., Meriluoto, J.S., Salminen, S. (2007). Role of commercial probiotic strains against human
pathogen adhesion to intestinal mucus. Lett. Appl. Microbiol, 45, 454-460.
[5] Dolgushin, I.I., Bukharin, O.V. (2001). Neutrophils and homeostasis. Ekaterinburg: Ural Division of Russian Academy of Sciences Press.
[6] Dotta, G., Pedreira, M.J.L., Jatobá, A., Morán, R.E.B., Pilati, C., Martins, M.L. (2011). Acute inflammatory response in Nile tilapia fed probiotic Lactobacillus plantarum in the diet. Acta Scientiarum. Biological Sciences, 33(3), 239-246.
[7] Ermolina, S.A., Ermolin, A.V., Zurochka, A.V. (2008). Phagocyte and lymphocyte links of the immune system in calves with nitroxidemia. Veterinary practice, 2(41), 26-29.
[8] Fedyukh, E.I., Polozyukh, O.N., Mikheeva, O.V., Fedyukh, V.V. (2016). Increase in the natural resistance of pigs. Pig breeding, 7, 25-27.
[9] Gamko, L.N., Chernenok, V.V., Chernenok, Y.N. (2010). Morphological and biochemical parameters of blood in young pigs fed with probiotics. Veterinary and feeding, 3, 10-11.
[10] Garkavi, L.Kh., Kvakina, E.B., Ukolova, M.A. (1990). Adaptational reactions and resistance of the organism. Rostov-on-Don: Rostov University Press.
[11] Gorlov, I.F., Slozhenkina, M.I., Baranikov, V.A., Nikolaev, D.V., Vodyannikov, V.I., Lodyanov, V.V.
(2019). Biochemical blood parameters in pigs during the evaluation of qualitative characteristics of meat.
Pig breeding, 1, 31-35.
[12] Kalashnikov, A.P., Ficinin, V.I., Scheglov, V.V., Pervoe, N.G., Kleimenov, N.I., Strekozov, N.I.,
Kalinitskiy, B.D., Egorov, I.A., Makhaev, E.A. et al. (2003). Norms and diet plans for agricultural
animals. Reference book, 3rd addition. Ministry of Agriculture of Russian Federation, Russian Academy of
Agricultural Sciences, All-Russian State Scientific Research Institute of Animal Breeding.
[13] Karland, J.I., Broxmeyer, H.E., Pelus, L.M. (1978). Role of monocyte-macrophage-derived colonystimulating factor and prostaglandin E in positive and negative freed-back control of myeloid stem cells
proliferation. Blood, 52, 388-497.
[14] Khaitov, R.B., Penegin, B.V., Istamov, Kh.I. (1995). Ecological immunology. М.: VNIRO.
[15] Kondrakhin, I.P., Arkhipov, A.V., Levchenko, V.I., Talanov, G.A., Frolova, L.A., Novikov, V.E. (2004).
Methods of veterinary clinical laboratory diagnostics. Reference book, М.: Koloss.
[16] Krapivina, E.V., Krivopushkin, A.V. (2009). Influence of time and dose of sodium nucleinate in vaccinated
pigs on microbicide activity of neutrophils in blood. Ecologic and breeding issues. Bryansk: BSAA Press.
[17] Lebedev, К.А., Ponyakina, I.D. (1990). Immunogramm in clinical practice. М.: Science.
[18] Makarevich, N.A. (1988). Lysosomal cation test for the evaluation of bovine organism resistance.
Veterinary, 5, 26-28.
[19] Maksimov, V.I., Verkhovskiy, O.A., Moskvina, A.S. (2014). Alterations in the blood picture in the bovine
cattle after vaccination. Veterinary, zootechny, and biotechnology, 8, 40-48.
[20] Marinin, E.A., Voroshilova, T.G., Masanska, V.V. (1993). Evaluation of group immunological reactivity of
young farm animals. Veterinary, 6, 10-12.
[21] Mayanskiy, A.N., Galiulin, A.N. (1984). Reactivity of neutrophils. Kazan: Kazan University Press.
[22] Mayanskiy, А.N., Mayanskiy, D.N. (1989). Neutrophils and macrophages. Novosibirsk: Nauka.
[23] Mazurik, V.K., Mikhailov V.F. (1999). Some molecular mechanisms of the main radiological
consequences of the ionizing radiation influence on mammals. Radiation biology. Radioecology, 39(1), 89-
96.
[24] McKean, J.D. (2012). Prudent use of antibiotics while reducing resistance. Pig Progr., 28(Spec. Ed.), 10-
11.
[25] Mevius, D. (2012). Resistance calls for changes in practice. Pig Progr, 28(Spec. Ed.), 8-9.
[26] Moshkutelo, I.I., Aleksandrov, P.V., Severin, V.P., Ryndina, D.F., Artemieva, O.A. (2010). Probiotic drugs
in the system of growing and feeding of piglets. Pig breeding, 2, 64-67.
[27] Nagaev, B.Е., Kantsaliev, L.B., Mezhchikhov, T.N. (1997). Intercellular components of the microbicide
system of neutrophils in patients with cholecystitis. Clinical laboratory diagnostics, 4, 3-5.
[28] Ovsyannikov, Y.S., Tikhonov, G.I., Golunova, O.V. (2009). Probiotics in veterinary. Veterinary medicine,
1-2, 66-68.
[29] Pavlov, D.S., Egorov, A.I., Nekrasov, R.V., Laktionov, K.S., Kravtsova, L.Z., Pravdin, V.G., Ushakova,
N.A. (2011). Biologically active feed supplements for the improvement of feeding properties of combined
feed and increase in the norms of introduction of oilcakes and millcakes. Issues of biology of productive
animals, 1, 89–92.
[30] Pavluykh, I.M. (1989). Stimulation of the resistance in pigs with dodetsoniy. Agricultural Issues, 11, 156-
158.
[31] Peixoto, F.B.S., Peixoto, J.C., Assunção, E.N., Peixoto, E.M., Pereira, J.O., Astolfi-Filho, S. (2017).
Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari.
Amazonas, 39(4), 489-496.
[32] Petrov, V.V., Bazylevskiy, A.A., Progor, A.V. (2012). Some parameters of the organism's status in healthy
piglets grown in the industrial conditions. Pig breeding, 3, 77-79.
[33] Petrovskiy, S.V., Khlebus, N.K., Sidorenko, A.O. (2013). Zootechnic and veterinary aspects of the increase
in the productivity of sows in the conditions of upscale industrial complexes: recommendations. Gorki:
BSAA.
[34] Plokhonskiy, N.A. (1961). Biometry. Issues of the Siberian Division of the Academy of Sciences.
[35] Rechkalova, T.G. (2006). Experience of application of microbiological drugs "EM-Kurunga" and “Baikal
EM1" and “Tamir” in pig breeding. Practical biotechnology in agriculture, ecology, and healthcare. Collection of works.
[36] Serova, V.V., Paunova, V.S. (1995). Inflammation. Guidelines for doctors. М.: Medicine.
[37] Shubich, M.G., Mednikova, V.G. (1978). Nitro Blue Tetrazolium Reduction Test in healthy children and in
children with septic-bacterial infections. Laboratory practice, 1, 663-666.
[38] Sidorov, Z.N. (2001). Application of EM technology in pig-breeding. Hope for the planet, 1, 12 -17.
[39] Silva, G.C., Soares, R.C., Carvalho, F.C.T., Rocha, R.S., Vieira, R.H., Sousa, O.V. (2018). Antibacterial
and cytotoxicity activity in macroalgae extracts: perspectives for the use against pathogenic bacteria from
shrimp farms (Litopenaeus vannamei). Biological Sciences, 40, e40053.
[40] Strom, K., Sjogren, J., Broberg, A., Schnurer, J. (2002). Lactobacillus plantarum MiLAB 393 produces the
antifungal cyclic dipeptides cyclo (L-Phe-L-Pro) and cyclo (LPhe-trans-4-OH-L-Pro) and 3- phenyllactic
acid. Appl. Environ. Microbiol, 68, 4322-4327.
[41] Sudakov, N.A., Bereza, V.I. (1987). Lysosome cation test on the nonspecific resistance in cows with
microelement insufficiency in cows. Veterinary, 1, 64-65.
[42] Uchasov, D.S., Yarovan, N.I., Sein, O.B. (2013). Efficiency of probiotic “provagen” in pigs with
technological stress. Issues of Orlov State Agricultural University, 1(40), 129-131.
[43] Vodyannikov, V.I., Shkalenko, V.V. (2017). Prevention of technological stress at the final stage of pigs
growing. Pig breeding, 2, 23-24.
[44] Voronina, О.А, Zaitsev, S.Y., Brylina, V.E., Belonovskaya, О.S. (2017). Evaluation of the activity
lysosome cation granulocyte proteins in animals with chronic kidney insufficiency. Veterinary, 5, 21-24.
[45] Wojcik, R. (2014). The effect of Bioleх-MB40 on the activity and oxidative metabolism of peripheral blood granulocytes and monocytes in lambs. Pol. J. Veter. Sc., 17(2), 281-291.
[46] Zhibinov, V.I. (1983). Application of lysosomal test. Veterinary, 8, 30-31.
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