Nano-ceftriaxone as Antibiofilm Agent

Authors

  • Alaa L. Abdulla Radiological Techniques Department /College of Health and Medical Technology- Iraq Author
  • Muhammed Mizher Radhi Radiological Techniques Department /College of Health and Medical Technology- Iraq Author
  • Intesar N. Khelkal Mustansiriyah University/College of science /Biology department-Iraq Author
  • Eman N. Naji Mustansiriyah University/College of science /Biology department-Iraq Author

DOI:

https://doi.org/10.61841/fckp2b22

Keywords:

Biofilm, antibiotics, ceftriaxone, nano- ceftriaxone

Abstract

Biofilms are communities of microorganisms that grow together and surrounded by an extracellular substance, which they form and prefer to adhere to living and non-living surfaces. Metals like copper, gold, silver, titanium, and zinc are known to have antibacterial and antibiofilm properties, which present alternatives to antibiotics without increasing the risk of resistance. The current study introduces the nano-form of ceftriaxone as agent has the activity to decrease biofilm production of some virulent bacterial isolates.

Ninety bacterial isolates have been collected from different clinical sources. Identification was confirmed by API 20E and VITEK 2 systems. Antimicrobial susceptibility towards 16 antibiotics and minimum inhibitory concentration (MICs) of ceftriaxone before and after conversion were determined by using the two-fold broth dilution method as a complementary test to verify the pattern of resistance.

MIC mean value of the nano-ceftriaxone was much less when compared with the MIC for microceftriaxone at the same concentration. Bacterial isolates under study included 12 MDR isolates have been screened for their ability to produce biofilms with and without different concentrations of micro and nanoceftriaxone ranges from (156-10000) µg/ml. The results in general showed highly variation in the biofilm formation degree as well as the reduction percentage after treatment with different concentrations of micro and nano-ceftriaxone.

 

 

Downloads

Download data is not yet available.

References

1. Shrestha LB, Bhattarai NR, KhanalB.( 2018): Comparative evaluation of methods for the detection of biofilm formation in coagulase- negative staphylococci and correlation with antibiogram. Infect Drug Resist.;11:607–13.

2. Ortega- Pena S and Hernandez-Zamora E (2018): Microbial biofilms and their impact on medical areas: physiopathology, diagnosis and treatment, Bol Med Hosp Infant Mex. 2018;75.

3. Brad M. D. Spellberg, G. John, M. D. Bartlett, N. David and M. D.Gilbert, N. ngl. J. Med.( 2013):The Future of Antibiotics and Resistance368, 299–302.

4. Ramachandran R and Sangeetha D ( 2017): Antibiofilm efficacy of silver nanoparticles against biofilm forming multidrug resistant clinical isolates.6(11): 36-43.

5. Arias C. A. and B. E. Murray(2012): The rise of the Enterococcus: beyond vancomycin resistance Nat. Rev. Microbiol., , 10, 266–278. aspects. Rev Rom Bioet 13: 423-432.

6. Jesline, A.; John, N.; Narayanan, P.; Vani, C.; Murugan, S.( 2014): Anti-microbial activity of zinc and titanium dioxide nanoparticles against biofilm-producing methicillin-resistant Staphylococcus aureus. Appl. Nanosci., 5.

7. Roy, A.; Parveen, A.; Koppalkar, A.R.; Prasad, M.( 2010): Effect of Nano—Titanium Dioxide with Different Antibiotics against Methicillin-Staphylococcus Aureus, Journal of Biomaterials and Nanobiotechnology, Vol.1 No. 1, pp. 37-41. doi: 10.4236/jbnb.2010.11005.

8. Naik K. and KowshikM.( 2014): Anti-biofilm efficacy of low temperature processed AgCl–TiO2 nanocomposite coating,” Materials Science and Engineering: C, vol. 34, no. 1, pp. 62–68.

9. Lee J.-H, Y.-G. Kim, M. H. Cho, and J. Lee.(2014): ZnO nanoparticles inhibit Pseudomonas aeruginosa biofilm formation and virulence factor production, Microbiological Research, vol. 169, no.12, pp. 888–896.

10. CLSI, (Clinical & Laboratory Standards institute). (2018): Performance standard for antimicrobial susceptibility testing.

11. Abdelwahed, W., Degobert, G., Stainmesse, S., &Fessi, H. (2006): Freeze-drying of nanoparticles: formulation, process and storage considerations. Advanced drug delivery reviews, 58(15), 1688-1713.

12. Hakim M Maqsood, Nazir Ahmad Ganai, Syed Mudasir Ahmad1, Oyas Ahmad Asimi, Tariq Raja, Feroz

Ahmad Shah, Jalal-ul-Din Parrah and Riaz Ahmad Shah (2019): Evaluation of In vitro Antioxidant Activity

of Nelumbo nucifera Leaf Extract and its Potential Application as Antibacterial Agent against Fish

Pathogens. Int.J.Curr.Microbiol.App.Sci 8(6): 379-389.

13. Géssica A. Costa, Fernanda C.P. Rossatto1, Aline W. Medeiros1, Ana Paula F. Correa2, Adriano Brandelli2, Ana Paula G. Frazzon1 And Amanda De S. Da Motta (2018): Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis An Acad Bras Cienc 90 (1).

14. Mathur S, Gutte M, Paul D, Udgire M. (2013): Study the effect of essential oils on microbial biofilm formation by Klebsiella pneumoniae. Sch Acad J Biosci 1:76-79.

15. Shao F ,Wen-Jie Ren, Wei-Zheng Meng, Gui-Zhi Wang, Tian-Yun Wang.(2018): Burn Wound Bacteriological Profiles, Patient Outcomes, and Tangential Excision Timing: A Prospective, observational study volume 64-issue 9 –September – issn 1943-2720.

16. PerweenN , S. Kirshna Prakash, and Oves Siddiqui (2015): Multi Drug Resistant Klebsiella Isolates in Burn Patients: A Comparative Study J Clin Diagn Res. Sep; 9(9): DC14–DC16.

17. MundhadaS G , Prakash H Waghmare, Prachala G Rathod, Kishore V Ingole (2015 )Bacterial and fungal

profile of burn wound infections in Tertiary Care Center Year : Volume : 23 , Issue : 1 , Page : 71-75.

18. McCarthy, M.( (2017): Woman dies after infection with bacteria resistant to all antibiotics S. BMJ 356, https: //doi.org/10. 1136/bmj .j254.

19. Santajit, S. &Indrawattana, N.( (2016): Mechanisms of Antimicrobial Resistance in ESKAPE Pathogens. BioMed Research International, 2475067.

20. Bhuiya M , Mohammad K. I. Sarkar, Mehadi H. Sohag, Hafij Ali,, Chapol K. Roy, LutfaAkther,and Abu F. Sarker.( 2018): Enumerating Antibiotic Susceptibility Patterns of Pseudomonas aeruginosaIsolated from Different Sources in Dhaka City The Open Microbiology Journal, , Volume 12 173.

21. Kumar S, Bhanjana G, Kumar A, Taneja K, Dilbaghi N, Kim KH.( 2016): Synthesis and optimization of ceftriaxone-loaded solid lipid nanocarriers Chem Phys Lipids. Oct;200:126-132. doi: 10.1016/j. chemphyslip.2016.09.002.

Downloads

Published

30.04.2020

Issue

Vol. 24 No. 2 (2020): 24 (2) 2020

Section

Articles

License

Copyright (c) 2020 AUTHOR

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

You are free to:

  1. Share — copy and redistribute the material in any medium or format for any purpose, even commercially.
  2. Adapt — remix, transform, and build upon the material for any purpose, even commercially.
  3. The licensor cannot revoke these freedoms as long as you follow the license terms.

Under the following terms:

  1. Attribution — You must give appropriate credit , provide a link to the license, and indicate if changes were made . You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  2. No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

Notices:

You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation .

No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.

How to Cite

L. Abdulla, A., Mizher Radhi, M., N. Khelkal, I., & N. Naji, E. (2020). Nano-ceftriaxone as Antibiofilm Agent. International Journal of Psychosocial Rehabilitation, 24(2), 195-205. https://doi.org/10.61841/fckp2b22