A LOCAL YOGURT WAS SUGGESTED TO BE AN ANTIMICROBIAL AGENT FOR TREATING ORAL INFECTION
DOI:
https://doi.org/10.61841/4hz6kr77Keywords:
Proteus mirabilis isolate from local yogurt,, s- antimicrobial agent.Abstract
A biological and chemical assays has been applied for studying the activity of an extracted active gradient from Iraqi (Kurdistan) local yogurt and it was used as antibacterial against pathogens isolated from oral infection. Gram (+ve) and gram (-ve) species were isolated and identified using Pincus protocol. The isolates preserved on nutrient agar at 370C and then cultured on a new culture media. Nutrient broth has been supplemented with 15% glycerol for long preservation so that the isolates maintained frozen for several months. Whey protein was precipitated from the local yogurt using 10% TCA solution. The precipitated protein shows a strong inhibitory effects on the isolates growth, the pathogen Proteus mirabilis.
Downloads
References
1. Guentzel MN (1996). Baron S; et al., eds. Escherichia, Klebsiella, Enterobacter, Serratia, Citrobacter, and Proteus. In: Barron's Medical Microbiology (4th ed.). Univ of Texas Medical Branch.
2. Bacteria of the species Proteus mirabilis are widely distributed in soil and water in the natural environment. In humans, Proteus is found as part of the normal flora of the gut from BioMedHTC
3. Proteus mirabilis and Urinary Tract Infection.
4. O'hara CM, Brenner FW, Miller JM. Classification, identification, and clinical significance of Proteus, Providencia, and Morganella. Clin Microbiol Rev. 2000;13(4):534-46.
5. Gonzalez, Gus; et al. "Proteus Infections Medication". Medscape. Retrieved 30 October 2015.
6. Marshall, K (2004). "Therapeutic applications of whey protein". Alternative Medicine Review. 9 (2): 136–
156.
7. Jay R. Hoffman & Michael J. Falvo (2004). "Protein - Which is best?" . Journal of Sports Science and Medicine (3): 118–130.
8. Luhovyy BL, Akhavan T, Anderson GH (2007). "Whey proteins in the regulation of food intake and satiety". Journal of the American College of Nutrition. 26 (6): 704S–712S.
9. Haug A, Høstmark AT, Harstad OM, A; Høstmark, AT; Harstad, OM (2007). "Bovine milk in human nutrition – a review". Lipids Health Dis. 6: 25.
10. David H. Pincus. Microbial Identification using the Biomerieux Vitek 2 system, USA (WWW.pda.org/bookstore).
11. Atlas RA. Handbook of Microbiological Media. CRC Press, Ann Arbor. 1993.
12. McFaddin,J.F. (2000) Biochemical test for identification of medical Bacteria.3rd edition.Lippincott Williams & Wilkins,Co. London. 1
13. Rasheed H.R, , Alwan S.S, , Salman D 2017, "Detection of aminoglycoside resistance gene aph(2")Ic in” clinical isolates of Enterococcus faecalis Volume: 13, Issue: 3, Part: 3 : 255-264
14. Chelikani P, Fita I, Loewen PC. 2004, ‘Diversity of structures and properties among catalases. Cell Mol Life Sci. 61(2):192-208.
15. Boon EM, Downs A, Marcey D. 2007, “Catalase: H2O2 Oxidoreductase. Catalase structural Tutorial Text, pp 02-11.
16. Poppitt Sd, Strike CM, McArdle BH, McGill A, 2013, “ Evidence of enhanced serum amino acid profile but not appetite suppression by dietary glycomacropeptide (GMP): a comparison of dairy whey proteins”. J Am Coll Nutr, 32(3):177-86.
17. Su, S.C., et al. (1996). Temperature variations in upright mechanical freezers. Cancer Epidemiol Biomarkers 5(2):139-40.
18. Finegold S.M, and Martin W.J, 1982 “Diagnostic Microbiology,” 6th Edition, C.V. Mosby Co. St. Louis, Toronto, London.
19. Etablering af et globalt salmonella-overvågningsnetværk: WHO global salm-surv.
20. Wegener, Henrik Caspar ; Petersen, A. ; Evans, M. C. ; Hendriksen, Rene S. ; Wong, D. M. A. Lo Fo
; Aarestrup, Frank Møller in: Alimenta, vol: 2, pages: 10-15, 2002
21. Shawna Marie Simpson,” Fate of pathogenic organisms in a whey beverage treated with high pressure processing”, 2012, An MSC, Thesis, Iowa State University Capstones, USA.
Downloads
Published
Issue
Section
License

This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format for any purpose, even commercially.
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
- The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit , provide a link to the license, and indicate if changes were made . You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Notices:
You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation .
No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.