The Effect of Ginger Honey and Cocktail Honey Supplementation on Cortisol Levels in Balb/c Female Mice Induced Stress
DOI:
https://doi.org/10.61841/4fgq8v21Keywords:
Ginger honey;, Cocktail honey, Cortisol, StressAbstract
This study aims to investigate the effect of ginger honey and cocktail honey on decreasing cortisol hormone levels and to determine the difference in the effect of ginger honey (GH) and cocktail honey (CH) on decreasing cortisol hormone. This study is an in vivo study using the pre-test-post-test control group method. The sample used in this study were 25 Balb/c female mice which were divided into 5 groups (each group consist 5 mices); 1 control group and 4 intervention groups. The Intervention group divided based on different combination for ginger honey and different dose for cocktail honey; GH I (28 mg/20g BW/day), GH II (28 mg/20g BW/day), CH I (14 mg/20g BW/day) and CH II (28 mg/20g BW/day). All of group were given oral intervention using oral sonde for 14 days. Cortisol levels were examined using the ELISA method. Data were analyzed using statistical analysis T-test paired test. In this study it was found that the group of GH II given for 14 days could significantly reduce cortisol levels (p= 0.005, p <0.05) by -1,418 ng/ml and CH II can significantly reduce cortisol hormone levels (p = 0.005, p
<0.05) by -0.806 ng/ml compared to the control group. However, among the two most influential on decreasing cortisol levels was GH II. This study concludes that the administration of ginger honey and cocktail honey can have an effect on reducing cortisol hormone levels and can be used as a supplement to deal with stress
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References
1. Holmqvist-Jämsén S., Johansson A., Santtila P., Westberg L., von der Pahlen B., Simberg S. Investigating the Role of Salivary Cortisol on Vocal Symptoms. J Speech, Lang Hear Res. 2017;60(10):2781-91, doi: 10.1044/2017_jslhr-s-16-0058.
2. Koolhaas JM., Bartolomucci A., Buwalda B., Boer SF De., Flügge G., Korte SM., et al. Neuroscience and Biobehavioral Reviews Stress revisited : A critical evaluation of the stress concept. Neurosci Biobehav Rev. 2011;35(5):1291-301, doi: 10.1016/j.neubiorev.2011.02.003.
3. Greff MJE., Levine JM., Abuzgaia AM., Elzagallaai AA., Rieder MJ., Uum SHM Van. Hair cortisol analysis: an update on methodological considerations and clinical applications. Clin Biochem. 2018, doi: 10.1016/j.clinbiochem.2018.09.010.
4. Lynch CD., Sundaram R., Buck Louis GM. Biomarkers of preconception stress and the incidence of pregnancy loss. Hum Reprod. 2018:1-8.
5. Lynch CD., Sundaram R., Maisog JM., Sweeney AM., Buck Louis GM. Preconception stress increases the risk
of infertility: results from couple-based prospective cohort study-the LIFE study. Hum Reprod. 2014;29(5):1067-75.
6. Qu F., Wu Y., Zhu Y., Barry J., Ding T., Baio G., et al. The association between psychological stress and miscarriage : A systematic review and meta-analysis. Sci Rep. 2017;(August 2016):1-8, doi: 10.1038/s41598- 017-01792-3.
7. Prasad S., Tyagi AK. Ginger and its constituents: Role in prevention and treatment of gastrointestinal cancer. Gastroenterol Res Pract. 2015;2015:1-11, doi: 10.1155/2015/142979.
8. Syafitri DM., Levita J., Mutakin M., Diantini A. A Review: Is Ginger (Zingiber officinale var. Roscoe) Potential for Future Phytomedicine? Indones J Appl Sci. 2018;8(1), doi: 10.24198/ijas.v8i1.16466.
9. Fadaki F., Modaresi M., Sajjadian I. The effects of ginger extract and diazepam on anxiety reduction in animal model. Indian J Pharm Educ Res. 2017;51(3):S159-62, doi: 10.5530/ijper.51.3s.4.
10. Khaki A., Khaki AA., Hajhosseini L., Golzar FS., Ainehchi N. The Anti-oxidant Effects of Ginger and Cinnamon on Spermatogenesis Dys-function of Diabetes Rats. Afr J Tradit Complement Altern Med. 2014;11(4):1-8.
11. Miguel MG., Antunes MD., Faleiro ML. Honey as a complementary medicine. Integr Med Insights. 2017;12:1-15, doi: 10.1177/1178633717702869.
12. Erejuwa OO., Sulaiman SA., Wahab MSA. Honey: A Novel Antioxidant. Molecules. 2012;(17):4400-23, doi: 10.3390/molecules17044400.
13. Erejuwa OO., Gurtu S., Sulaiman SA., Ab Wahab MS., Sirajudeen KN., Salleh MS. Hypoglicemic and antioxidant effects of honey supplementation in streptozotocin-induced diabetic rats. Int J Vitam Nutr Res. 2010;(80):74-82.
14. Pasupuleti VR., Sammugam L., Ramesh N., Gan SH. Honey, Propolis, and Royal Jelly: A Comprehensive Review of Their Biological Actions and Health Benefits. Oxid Med Cell Longev. 2017;2017:1-21, doi: 10.1155/2017/1259510.
15. Azman KF., Zakaria R., Abdul Aziz CB., Othman Z. Tualang Honey Attenuates Noise Stress-Induced Memory Deficits in Aged Rats. Oxid Med Cell Longev. 2016;2016, doi: 10.1155/2016/1549158.
16. Maqsoudlou A., Mahoonak AS., Mora L., Mohebodini H., Toldrá F., Ghorbani M. Peptide identification in alcalase hydrolysated pollen and comparison of its bioactivity with royal jelly. Food Res Int. 2019;116(September):905-15, doi: 10.1016/j.foodres.2018.09.027.
17. Kocot J., Kiełczykowska M., Luchowska-Kocot D., Kurzepa J., Musik I. Antioxidant Potential of Propolis, Bee Pollen, and Royal Jelly: Possible Medical Application. Oxid Med Cell Longev. 2018;2018:1-29, doi: 10.1155/2018/7074209.
18. Asama T., Matsuzaki H., Fukushima S., Tatefuji T., Hashimoto K., Takeda T. Royal Jelly Supplementation Improves Menopausal Symptoms Such as Backache, Low Back Pain, and Anxiety in Postmenopausal Japanese Women. Evidence-Based Complement Altern Med. 2018;2018:1-7, doi: 10.1155/2018/4868412.
19. Habryka C., Kruczek M. Bee products used in apitherapy. 2016;(May).
20. Bakour M., Fernandes Â., Barros L., Sokovic M., Ferreira ICFR., Badiaa lyoussi. Bee bread as a functional product: Chemical composition and bioactive properties. Lwt-Food Sci Technol. 2019;109:276-82, doi: 10.1016/j.lwt.2019.02.008.
21. Kieliszek M., Piwowarek K., Kot AM., Błażejak S., Chlebowska-Śmigiel A., Wolska I. Pollen and bee bread as new health-oriented products: A review. Trends Food Sci Technol. 2018;71:170-80, doi: 10.1016/j.tifs.2017.10.021.
22. Hannibal KE., Bishop MD. Chronic Stress, Cortisol Dysfunction, and Pain: A Psychoneuroendocrine Rationale for Stress Management in Pain Rehabilitation. Phys Ther. 2014;94(12):1816-25, doi: 10.2522/ptj.20130597.
23. Jones JH. Resource Book for the Design of Animal Exercise Protocols. Am J Vet Res. 2007;68(6):583-583, doi: 10.2460/ajvr.68.6.583.
24. Mosavat M., Ooi FK., Mohamed M. Stress hormone and reproductive system in response to honey supplementation combined with different jumping exercise intensities in female rats. Biomed Res Int. 2014;2014, doi: 10.1155/2014/123640.
25. Khosravi T., Modaresi M., Sajjadian I. Comparing the Effects of Ginger and Imipramine on Reducing Depression Symptoms of Animal Model. Indo Am J Pharm Sci. 2018;5(1):37-41, doi: 10.5281/zenodo.1135269.
26. Büyükipekçi S., Sarıtaş N., Soylu M., Mıstık S., Silici S. Effects of royal jelly and honey mixture on some hormones in young males performing maximal strength workout. Phys Educ students. 2018;22(6):308-15, doi: 10.15561/20755279.2018.0605.
27. Thej, M.J., Kalyani, R., Kiran, J.Atherosclerosis in coronary artery and aorta in a semi-urban population by applying modified American Heart Association classification of atherosclerosis: An autopsy study(2012) Journal of Cardiovascular Disease Research, 3 (4), pp. 265-271.
28. Dudi, B., Rajesh, V.An efficient algorithm for medicinal plant recognition(2018) International Journal of Pharmaceutical Research, 10 (3), pp. 87-93.
29. Pundir, S., Amala, R.A study on the Bi-Rayleigh ROC model(2012) Bonfring International Journal of Data Mining, 2 (2), pp. 42-47.
30. Fattahi, S., Naderi, F., Asgari, P., Ahadi, H. Neuro-feedback training for overweight women: Improvement of food craving and mental health (2017) NeuroQuantology, 15 (2), pp. 232-238.
31. B.Mahalakshmi, G.Suseendran “Effectuation Of Secure Authorized Deduplication in Hybrid Cloud” Indian Journal of Science and Technology, Vol(9(25), July 2016. pp.1-7. DOI: 10.17485/ijst/2016/v9i25/87990
32. Syed Jawad Hussain, Sohail Maqsood, NZ Jhanjhi, Azeem Khan, Mahadevan Supramaniam and Usman Ahmed, “A Comprehensive Evaluation of Cue-Words based Features and In-text Citations based Features for Citation Classification” In International Journal of Advanced Computer Science and Applications(IJACSA), 10(7), pp. 209-218. 2019
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