INFLUENCE OFDIFFERENTCONCENTRATION OF CURCUMIN (DIFERUOYL METHANE) ON FROZEN SEMEN QUALITY OF HOLSTEIN BULLS BORN IN IRAQ
DOI:
https://doi.org/10.61841/kc3nws07Keywords:
curcumin, frozen semen, Holstein bulls, IraqAbstract
Object: The current study was designed to improve semen preservability through the use of curcumin (diferuoyl methane) in different concentrations (T1 0.5, T2 1.0, and T3 1.5 mM/ml) and control (without additive) as extender additive expressed in frozen bull semen .
Methods: Ejaculates with less than 40% of initial individual motility were taken weekly from (3) Holstein bulls born in Iraq by using the artificial vagina. The semen samples were diluted with Tris containing different concentrations of curcumin (0.5, 1.0, 1.5 mM/ml) and control (without additive), cooled slowly up to 5°C, and equilibrated for 4 hours. The semen was packed into 0.25 ml straws and frozen and storage hours in liquid nitrogen. Evaluated semen for individual motility%,d (%), abnormality (%), acrosome integrity% and HOST%.at steps of freezing (after cooling and post-thawing).
Results: Clearly indicated that the addition of 1.5 mM of curcumin to Tris diluents had significantly (P < 0.05) higher individual motility %, acrosome integrity% and HOST test % and less (P < 0.05) sperm dead %, abnormality % in comparison to the 0.5 mM of curcumin and control group. Besides, the addition of 1.0 mM of curcumin also has a promising effect when added to the tris-base diluents, while the addition of 0.5 mM of curcumin had little or no effect on all properties, which studies of cooled dilution semen and frozen in liquid nitrogen. In addition, the present study revealed that post-thawing had a clear effect, as it led to a significant (P < 0.05) decrease in the individual motility%,acrosoma integrity %, and HOS test % of sperm. On the other hand, it also caused an increase in dead sperm % and abnormal sperm % compared with after cooling, but the addition of curcumin in concentration1.5 mM/ml caused a decrease in this effect of freezing on some sperm properties, such as individual motility % and abnormality % of sperm.
Conclusion: Adding curcumin with a concentration of 1.5 mM/ml to Tris diluent caused improved poor sperm properties of bulls and decreased the effect of freezing on these samples.
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