The Effect of Ginger Honey and Cocktail Honey Supplementation on Glutathione Levels in Balb/C Female Mice Induced Stress
DOI:
https://doi.org/10.61841/mvb1hx94Keywords:
- Glutathione levels, Ginger honey, Cocktail Honey, ; Balb/c female miceAbstract
This study aims to determine the effect of Ginger honey and Cocktail honey supplementation on increasing glutathione levels and to assess the difference in the effect of Ginger honey and Cocktail honey administration on increasing glutathione levels in female Balb/c mice. The intervention in this research is conducted at the Biopharmaca Laboratory of the Faculty of Pharmacy, Hasanuddin University, and the examination of glutathione levels is carried out at the Microbiology Laboratory of Hasanuddin University Education hospital. The method of this research is in vivo research with the pretest-post-test control group. The samples in this study are 25 female Balb/c mice and are divided into five groups (each 5) with one positive control group (swimming activity) and four intervention groups. The provision of intervention in the form of ginger honey is as much as 28 mg/gramBW sample once every day for 14 days. The provision of cocktail honey (containing honey, royal jelly, and bee bread) as for the division of groups is distinguished from the administration dose of 14 mg/gramBW a day on cocktail honey I and 28 mg/ gramBW a day on cocktail honey II. Glutathione levels are examined using the ELISA method, statistical analysis using paired sample T-test. In this research, it is found that administration of ginger honey II containing 33% honey and extract ginger at a dose 28 mg/gramBW (p = 0.031) and cocktail honey II at a dose of 28 mg/gramBW (p = 0.044) given once a day for 14 days can significantly increase glutathione levels with a value of p <0.05. This study concludes that the administration of ginger honey and cocktail honey have an effect on the increase in glutathione levels, which are statistically significant
Downloads
References
[1] Abdul Sani, Nur Fathiah et al. 2014. “Effect of the Combination of Gelam Honey and Ginger on Oxidative Stress and Metabolic Profile in Streptozotocin-Induced Diabetic Sprague-Dawley Rats.” BioMed Research International 2014(Dm).
[2] Abdulmajeed, Wahab Imam, Habeeb Bolakale Sulieman, Sikiru Abayomi Biliaminu, and Lukuman Aboyeji Oyewole. 2015. “Honey Prevents Neurobehavioural Deficit and Oxidative Stress Induced by Lead Acetate Exposure in Male Wistar Rats- a Preliminary Study.”
[3] Abolaji, Amos O et al. 2017. “Protective Properties of 6-Gingerol-Rich Fraction from Zingiber Officinale (Ginger) on Chlorpyrifos-Induced Oxidative Damage and Inflammation in the Brain, Ovary and Uterus of Rats.” Chemico-Biological Interactions. http://dx.doi.org/10.1016/j.cbi.2017.03.017.
[4] Adeoye, Oyewopo, Johnson Olawumi, Adeleke Opeyemi, and Oyewopo Christiania. 2018. “Review Article Review on the Role of Glutathione on Oxidative Stress and Infertility.” 22(1): 61–66.
[5] Ahmed, Sarfraz et al. 2018. “Review Article Honey as a Potential Natural Antioxidant Medicine : An Insight into Its Molecular Mechanisms of Action.” 2018.
[6] Almasaudi, Saad B. et al. 2016. “Antioxidant, Anti-Inflammatory, and Antiulcer Potential of Manuka Honey against Gastric Ulcer in Rats.” Oxidative Medicine and Cellular Longevity 2016.
[7] Asadi, Nematollah et al. 2019. “Effect of Royal Jelly on Testicular Antioxidant Enzymes Activity, MDA Level and Spermatogenesis in Rat Experimental Varicocele Model.” Tissue and Cell 57(February): 70–77. https://doi.org/10.1016/j.tice.2019.02.005.
[8] Bilski, Jan et al. 2019. “Effect of Forced Physical Activity on the Severity of Experimental Colitis in Normal Weight and Obese Mice. Involvement of Oxidative Stress and Proinflammatory Biomarkers.” Nutrients 11(5).
[9] Caixeta, Douglas Carvalho et al. 2018. “Adaptogenic Potential of Royal Jelly in Liver of Rats Exposed to Chronic Stress.” PLoS ONE 13(1): 1–13
[10] Erejuwa, Omotayo O, Siti A Sulaiman, and Mohd S Ab Wahab. 2012. “Honey: A Novel Antioxidant.” : 4400–4423.
[11] Erejuwa, Omotayo Owomofoyon et al. 2011. “Comparison of Antioxidant Effects of Honey, Glibenclamide, Metformin, and Their Combinations in the Kidneys of Streptozotocin-Induced Diabetic Rats.” International Journal of Molecular Sciences 12(1): 829–43.
[12] Gu, Hyejung et al. 2018. “Antioxidant Activity of Royal Jelly Hydrolysates Obtained by Enzymatic Treatment.” Korean Journal for Food Science of Animal Resources 38(1): 135–42.
[13] Karadeniz, Ali et al. 2011. “Royal Jelly Modulates Oxidative Stress and Apoptosis in Liver and Kidneys of Rats Treated with Cisplatin.” 2011.
[14] Khalil, M I, S A Sulaiman, and L Boukraa. 2010. “Antioxidant Properties of Honey and Its Role in Preventing Health Disorder.” : 6–16.
[15] Kruk, Joanna, Hassan Y. Aboul-Enein, Aleksandra Kładna, and Jacquelyn E. Bowser. 2019. “Oxidative Stress in Biological Systems and Its Relation with Pathophysiological Functions: The Effect of Physical Activity on Cellular Redox Homeostasis.” Free Radical Research 53(5): 497–521. https://doi.org/10.1080/10715762.2019.1612059.
[16] Lingga, Lanny. 2012. The Healing Power of Antioxidant. Jakarta: PT Gramedia.
[17] Miguel, M G, and M L Faleiro. 2017. “Honey as a Complementary Medicine.”
[18] Pizzorno, Joseph. 2014. “The Path A Head, Glutathione.” Integrative Medicine • 13(1): 8–12. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4684116/pdf/8-12.pdf.
[19] Suhartono, Eko. 2016. Toksisitas Oksigen Reaktif & Antioksidan Di Bidang Kedokteran Dan Kesehatan. Yogyakarta: Gosyen Publishing.
[20] Teixeira, Renata Roland et al. 2017. “Royal Jelly Decreases Corticosterone Levels and Improves the Brain Antioxidant System in Restraint and Cold Stressed Rats.” Neuroscience Letters 655: 179–85. http://dx.doi.org/10.1016/j.neulet.2017.07.010.
[21] Yan, Huiru, Haihong Jia, and Hongru Gao. 2013. “Identification , Genomic Organization , and Oxidative Stress Response of a Sigma Class Glutathione S-Transferase Gene ( AccGSTS1 ) in the Honey Bee , Apis Cerana Cerana.” : 415–26.
[22] Abu. Marrzoq, L.F., Sharif, F.A., Abed, A.A.Relationship between ApoE gene polymorphism and coronary heart disease in Gaza Strip(2011) Journal of Cardiovascular Disease Research, 2 (1), pp. 29-35.
DOI: 10.4103/0975-3583.78584
[23] Firas Hasan Bazzari. "Available Pharmacological Options and Symptomatic Treatments of Multiple Sclerosis." Systematic Reviews in Pharmacy 9.1 (2018), 17-21. Print. doi:10.5530/srp.2018.1.4
[24] Vaibhav walia (2016) role of enzymes in the pathogenesis of depression. Journal of Critical Reviews, 3 (2), 11-16.
[25] Selvakumar, S., Chandrasekar, N., Magesh, N.S.Preliminary investigation of groundwater quality along the coastal aquifers of southern Tamil Nadu using GIS techniques(2012) Bonfring Int J Ind Eng Manag Sci, 2 (1), pp. 46-52.
[26] Vimal, R.L.P. Towards a Theory of Everything Part II Introduction of consciousness in Schrödinger equation and standard model (2010) NeuroQuantology, 8 (3), pp. 304-313.
[27] K.Rohini, G.Suseendran, “Aggregated K Means Clustering and Decision Tree Algorithm for Spirometry Data”, Indian Journal of Science and Technology, Volume 9, Issue 44, November 2016 pp.1-6 DOI:
[28] K Hussain, NZ Jhanjhi, H Mati-ur-Rahman, J Hussain, MH Islam, Using a systematic framework to critically analyze proposed smart card based two factor authentication schemes, Journal of King Saud University-Computer and Information Sciences
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format for any purpose, even commercially.
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
- The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit , provide a link to the license, and indicate if changes were made . You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Notices:
You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation .
No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.
