The Ginger Honey And Cocktail Honey Supplementation Effect On Estradiol Hormone Levels In Female Balb/C Mice Stress-Induced Through Swimming Activities
DOI:
https://doi.org/10.61841/nw3f2h22Keywords:
Estradiol levels;, Ginger Honey;, Cocktail Honey;, Reproductive Hormone, female Balb/c mic, swimming activitiesAbstract
This study intends to determine the effect of Ginger Honey and Cocktail Honey on Estradiol (E2) hormone levels, and to assess the magnitude of the difference in the effects of Ginger Honey and Cocktail Honey on Estradiol levels in female Balb/c mice induced stress. A total of 25 female Balb/c mice were used in this study and were divided into 4 groups (5 in each group) with 1 control group (negative control), 2 intervention groups Ginger Honey: Ginger Honey I (containing 50% Honey: 50% ginger extract), Ginger Honey II (33% Honey: 67% ginger extract) a dose of 28mg/20gBW of mice, and Cocktail Honey Group (trigona spp. honey, royal jelly and bee bread) with the distribution of Cocktail Honey I (14mg/20gBW), Cocktail Honey II (28mg/20gBW) given 1 time/day for 14 days. Estradiol levels were examined using the ELISA kit mouse. This study was an in vivo laboratory test with a pretest-posttest design with a control group. The data were displayed as the mean and analyzed using a paired T- test. This study shows that the intervention of Ginger Honey I (50% Honey: 50% ginger extract), Ginger Honey II (33% Honey: 67% ginger extract) at a dose of 28mg/20gBW of mice, and Cocktail Honey (honey, royal jelly and bee bread) with a dose distribution Cocktail Honey I (14mg/20gBW), Cocktail Honey II (28mg/20gBW) have a positive effect and statistically have a significant value on the levels of Estradiol in female Balb/c mice induced stress when compared to control group. The statistical results both shows P<0.05. However, between both, the greatest effect on increasing levels of Estradiol is shown by the Cocktail Honey I group of 19,466 ng/ml. This study concluded that among all the intervention groups, both Ginger Honey and Cocktail Honey has great influence on the hormone Estradiol. However, Cocktail Honey I has the greatest effect on Estradiol hormone levels.
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[1] Ryan KJ. Biochemistry of Aromatase: Significance to Female Reproductive Physiology. aacrjournals. 1982;42(8).
[2] Sherwood L. FISIOLOGI MANUSIA : DARI SEL KE SISTEM. En: Ong HO, Mahode AA, Ramdhani D,
editores. 8.a ed. Jakarta: ECG; 2016.
[3] Ray JA., Kushnir MM., Bunker A., Rockwood AL., Meikle AW. Direct measurement of free estradiol in human serum by equilibrium dialysis-liquid chromatography-tandem mass spectrometry and reference intervals of free estradiol in women. Clin Chim Acta. 2012;413(11-12):1008-14.
[4] Jasienska G. Lifestyle, hormones, and risk of breast cancer. TT -. BMJ Br Med J. 2001;322(7286):586-7.
[5] Rosner W., Hankinson SE., Sluss PM., Vesper HW., Wierman ME. Challenges to the measurement of estradiol: An endocrine society position statement. J Clin Endocrinol Metab. 2013;98(4):1376-87, doi: 10.1210/jc.2012-3780.
[6] Yager JD., Davidson NE. Estrogen carcinogenesis in breast cancer N Engl J Med 354 (3): 270–282. Find this Artic online. 2006:270-82.
[7] Yu H., Shu XO., Shi R., Dai Q., Jin F., Gao YT., et al. Plasma sex steroid hormones and breast cancer risk in Chinese women. Int J Cancer. 2003;105(1):92-7, doi: 10.1002/ijc.11034.
[8] Popat VB., Calis KA., Vanderhoof VH., Cizza G., Reynolds JC., Sebring N., et al. Bone mineral density in estrogen-deficient young women. J Clin Endocrinol Metab. 2009;94(7):2277-83, doi: 10.1210/jc.2008- 1878.
[9] Soyupek F., Ayhan F., Ceceli E., Yorgancioglu R. The evaluation of hand function in women: The important role of estrogen. J Back Musculoskelet Rehabil. 2006;19(4):103-9, doi: 10.3233/BMR-2006- 19401.
[10] Barut MU., Çoksüer H., Sak S., Bozkurt M., Ağaçayak E., Hamurcu U., et al. Evaluation of Sexual Function in Women with Hypogonadotropic Hypogonadism Using the Female Sexual Function Index (FSFI) and the Beck Depression Inventory (BDI). Med Sci Monit. 2018;24:5610-8, doi: 10.12659/msm.910304.
[11] Zaid SSM., Sulaiman SA., Sirajudeen KNM., Othman NH. The effects of tualang honey on female reproductive organs, tibia bone and hormonal profile in ovariectomised rats - animal model for menopause. BMC Complement Altern Med. 2010;10, doi: 10.1186/1472-6882-10-82.
[12] Trivedi R., Kumar S., Kumar A., Siddiqui JA., Swarnkar G., Gupta V., et al. Kaempferol has osteogenic effect in ovariectomized adult Sprague-Dawley rats. Mol Cell Endocrinol. 2008;289(1-2):85-93, doi: 10.1016/j.mce.2008.02.027.
[13] Henderson K., Aldhirgham T., Nigam P., Owusu-Apenten R. Evaluation of Manuka Honey Estrogen Activity Using the MCF-7 Cell Proliferation Assay. J Adv Biol Biotechnol. 2016;10(3):1-11, doi: 10.9734/jabb/2016/29887.
[14] Moutsatsou P., Papoutsi Z., Kassi E., Heldring N., Zhao C., Tsiapara A., et al. Fatty acids derived from royal jelly are modulators of estrogen receptor functions. PLoS One. 2010;5(12):1-10, doi: 10.1371/journal.pone.0015594.
[15] Suzuki KM., Isohama Y., Maruyama H., Yamada Y., Narita Y., Ohta S., et al. Estrogenic activities of fatty acids and a sterol isolated from royal jelly. Evidence-based Complement Altern Med. 2008;5(3):295-302, doi: 10.1093/ecam/nem036.
[16] Takahashi Y. Molecular alterations during female reproductive aging: Can aged oocytes remind youth? Embryol - Updat Highlights Class Top. 2012, doi: 10.5772/35938.
[17] Waykar B., Alqadhi YA. Indian Journal of Pharmaceutical and Biological Research ( IJPBR ) Biological Properties and Uses of Honey : A Concise Scientific review. 2016;4(3):58-68.
[18] Kieliszek M., Piwowarek K., Kot AM., Błażejak S., Chlebowska-Śmigiel A., Wolska I. Pollen and bee bread as new health-oriented products: A review. Trends Food Sci Technol. 2018;71:170-80, doi: 10.1016/j.tifs.2017.10.021.
[19] Oh J., Jo H., Cho AR., Kim SJ., Han J. Antioxidant and antimicrobial activities of various leafy herbal teas.
Food Control. 2013;31(2):403-9, doi: 10.1016/j.foodcont.2012.10.021.
[20] Atashpour S., Kargar Jahromi H., Kargar Jahromi Z., Maleknasab M. Comparison of the effects of Ginger extract with clomiphene citrate on sex hormones in rats with polycystic ovarian syndrome. Int J Reprod Biomed. 2017;15(9):561-8, doi: 10.29252/ijrm.15.9.561.
[21] Mokhtari M., Reza ME., Harfsheno S. The effects of alcoholic extract of Marrubum vulgare on hormonal parameters in female rat model of polycystic ovarian syndrome. Med Sci. 2014;24(2):74-80.
[22] Fadaki F., Modaresi M., Sajjadian I. The Effects of Ginger Extract and Diazepam on Anxiety Reduction in Animal Model. 2017;51(3):159-62, doi: 10.5530/ijper.51.3s.4.
[23] Rhode J., Fogoros S., Zick S., Wahl H., Griffith KA., Huang J., et al. BMC Complementary and Ginger inhibits cell growth and modulates angiogenic factors in ovarian cancer cells. 2007;9:1-9, doi: 10.1186/1472-6882-7-44.
[24] Sook H., Young E., Kang NE., Kyung W. [ 6 ] -Gingerol inhibits metastasis of MDA-MB-231 human breast cancer cells. 2008;19:313-9, doi: 10.1016/j.jnutbio.2007.05.008.
[25] Contarteze RVL., Manchado FDB., Gobatto CA., De Mello MAR. Biomarkers of stress in rats exercised in swimming at intensities equal and superior to the maximal estable lactate phase. Rev Bras Med do Esporte. 2007;13(3):169-74, doi: 10.1590/S1517-86922007000300008.
[26] Frolich MA, Banks C, Warren W, Robbins M NT. The Association Between Progesterone, Estradiol, and Oxytocin and Heat Pain Measures in Pregnancy: An Observational Cohort Study. Anesth Analg. 2016;123(2):396-402, doi: 10.1213/ANE.0000000000001259.
[27] Olive DL. Editorial: Exercise and fertility: An update. Curr Opin Obstet Gynecol. 2010;22(4):259-63, doi: 10.1097/GCO.0b013e32833c7227.
[28] Roosevelt SL. in Swimmers : A Distinct. 2014;(July):2740-4.
[29] Jana K., Dutta A., Chakraborty P., Manna I., Firdaus SB., Bandyopadhyay D., et al. Alpha-lipoic acid and N-acetylcysteine protects intensive swimming exercise-mediated germ-cell depletion, pro-oxidant generation, and alteration of steroidogenesis in rat testis. Mol Reprod Dev. 2014;81(9):833-50, doi: 10.1002/mrd.22354.
[30] Nazanin S., Saadat S., Student MD., Mohammadghasemi F., Sajedi HR., Student MD., et al. Ovarian and uterine alterations following forced swimming: An immunohistochemical study. 2016;14(10):629-36.
[31] Al-Rahbi B., Zakaria R., Othman Z., Hassan A., Ahmad AH. Protective Effects of Tualang Honey against Oxidative Stress and Anxiety-Like Behaviour in Stressed Ovariectomized Rats. Int Sch Res Not. 2014;2014:1-10, doi: 10.1155/2014/521065.
[32] Aschbacher K., O’Donovan A., Wolkowitz OM., Dhabhar FS., Su Y., Epel E. Good Stress, Bad Stress and Oxidative Stress: Insights from Anticipatory Cortisol Reactivity. Psychoneuroendocrinology. 2014;38(9):1698–1708, doi: 10.1016/j.psyneuen.2013.02.004.
[33] Mishima S., Suzuki KM., Isohama Y., Kuratsu N., Araki Y., Inoue M., et al. Royal jelly has estrogenic effects in vitro and in vivo. J Ethnopharmacol. 2005;101(1-3):215-20, doi: 10.1016/j.jep.2005.04.012.
[34] Ahmed S., Othman NH., Shamim N., Baig AA., Jabeen S., Liaqat S., et al. Honey as a Potential Natural Antioxidant Medicine: An Insight into Its Molecular Mechanisms of Action. Oxid Med Cell Longev. 2018;2018:1-19, doi: 10.1155/2018/8367846.
[35] Erejuwa OO., Sulaiman SA., Wahab MSA., Sirajudeen KNS., Salleh MSM., Gurtu S. Differential responses to blood pressure and oxidative stress in streptozotocin-induced diabetic wistar-kyoto rats and spontaneously hypertensive rats: Effects of antioxidant (Honey) treatment. Int J Mol Sci. 2011;12(3):1888- 907, doi: 10.3390/ijms12031888.
[36] Petelin A., Kenig S., Kopinč R., Deželak M., Černelič Bizjak M., Jenko Pražnikar Z. Effects of royal jelly administration on lipid profile, satiety, inflammation, and antioxidant capacity in asymptomatic overweight adults. Evidence-based Complement Altern Med. 2019;2019, doi: 10.1155/2019/4969720.
[37] Mumford SL., Browne RW., Schliep KC., Schmelzer J., Plowden TC., Michels KA., et al. Serum Antioxidants Are Associated with Serum Reproductive Hormones and Ovulation among Healthy Women. J Nutr. 2016;146(1):98-106, doi: 10.3945/jn.115.217620.
[38] Azman KF., Zakaria R., Abdul Aziz CB., Othman Z. Tualang Honey Attenuates Noise Stress-Induced Memory Deficits in Aged Rats. Oxid Med Cell Longev. 2016;2016, doi: 10.1155/2016/1549158.
[39] Ghasemzadeh A., Jaafar HZE., Rahmat A. Antioxidant activities, total phenolics and flavonoids content in two varieties of malaysia young ginger (Zingiber officinale Roscoe). Molecules. 2010;15(6):4324-33, doi: 10.3390/molecules15064324.
[40] Danciu C., Vlaia L., Fetea F., Hancianu M., Coricovac DE., Ciurlea SA., et al. Evaluation of phenolic profile, antioxidant and anticancer potential of two main representants of Zingiberaceae family against
B164A5 murine melanoma cells. Biol Res. 2015;48:1-9, doi: 10.1186/0717-6287-48-1.
[41] Ha SK., Moon E., Ju MS., Kim DH., Ryu JH., Oh MS., et al. 6-Shogaol, a ginger product, modulates neuroinflammation: A new approach to neuroprotection. Neuropharmacology. 2012;63(2):211-23, doi: 10.1016/j.neuropharm.2012.03.016.
[42] Dugasani S., Pichika MR., Nadarajah VD., Balijepalli MK., Tandra S., Korlakunta JN. Comparative antioxidant and anti-inflammatory effects of [6]-gingerol, [8]-gingerol, [10]-gingerol and [6]-shogaol. J Ethnopharmacol. 2010;127(2):515-20, doi: 10.1016/j.jep.2009.10.004.
[43] Kundu JK., Surh YJ. Molecular basis of chemoprevention with dietary phytochemicals: Redox-regulated transcription factors as relevant targets. Phytochem Rev. 2009;8(2):333-47, doi: 10.1007/s11101-009-9132- x.
[44] Akinyemi AJ., Ademiluyi AO., Oboh G. Aqueous extracts of two varieties of ginger (Zingiber officinale) inhibit angiotensin I-Converting enzyme, iron(II), and sodium nitroprusside-induced lipid peroxidation in the rat heart in vitro. J Med Food. 2013;16(7):641-6, doi: 10.1089/jmf.2012.0022.
[45] Ji K., Fang L., Zhao H., Li Q., Shi Y., Xu C., et al. Ginger Oleoresin Alleviated γ -Ray Irradiation-Induced Reactive Oxygen Species via the Nrf2 Protective Response in Human Mesenchymal Stem Cells. Oxid Med Cell Longev. 2017;2017, doi: 10.1155/2017/1480294.
[46] Liu J., Burdette JE., Sun Y., Deng S., Schlecht SM., Zheng W., et al. Isolation of linoleic acid as an estrogenic compound from the fruits of Vitex agnus-castus L. (chaste-berry). Phytomedicine. 2004;11(1):18-23, doi: 10.1078/0944-7113-00331.
[47] Yao, L., Romero, M.J., Toque, H.A., Yang, G., Caldwell, R.B., Caldwell, R.W. The role of RhoA/Rho kinase pathway in endothelial dysfunction (2010) Journal of Cardiovascular Disease Research, 1 (4), pp. 165-170.
[48] Ozaifa kareem, nasir nisar, nahida tabassum (2017) mu-opioid receptor antagonists and their role in treatment of chronic constipation. Journal of Critical Reviews, 4 (5), 1-6. doi:10.22159/jcr.2017v4i5.22260
[49] Murthy, H.S.N., Meenakshi, D.M., Babaeizadeh, S., Laciar, E.ANN, SVM and KNN classifiers for prognosis of cardiac ischemia-A comparison(2015) Bonfring International Journal of Research in Communication Engineering, 5 (2), pp. 7-11.
[50] Hameroff, S.Orchestrated reduction of quantum coherence in brain icrotubules: A model for consciousness (2007) NeuroQuantology, 5 (1), pp. 1-8.
[51] B.Mahalakshmi, G.Suseendran “An Analysis of Cloud Computing Issues on Data Integrity, Privacy and its Current Solutions”, Editor V. E. Balas et al. (eds.), Data Management, Analytics and Innovation, Advances in Intelligent Systems and Computing 839, 2019, pp.467-482, Doi: 10.1007/978-981-13-1274-8_35
[52] Dr. Jafar A. Alzubi, Dr. Omar A. Alzubi, Dr.G.Suseendran, Dr.D.Akila “+A novel Chaotic map encryption methodology for image cryptography and secret Communication with steganography- International Journal of Recent Technology and Engineering, Vol.8(1C2), May 2019, pp.1122-1128
[53] C. Diwaker, P. Tomar, A. Solanki, A. Nayyar, NZ Jhanjhi, et al., "A New Model for Predicting Component-Based Software Reliability Using Soft Computing," in IEEE Access, vol. 7, pp. 147191- 147203, 2019. doi: 10.1109/ACCESS.2019.2946862
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