Molecular Detection of IntegronclassI Gene in Pseudomonas Aerginosa Isolated from Otitis Media
DOI:
https://doi.org/10.61841/hg8s8s10Keywords:
P.aerginosa, IntclassI, Otitis MediaAbstract
The study aimed to investigate the Pseudomonas aeruginosa from patients suffering from otitis media infection. The study included the bacterial diagnosis based on reliable diagnostic procedures. A total of 40 specimens were collected from ear swabs in Al-Najaf province within the period from September to December 2019. These patients ranged from 30 to 75 years. The male more than the female. Primary identification depended on Gram staining and biochemical tests. Finally, identification with the Vitek 2 system was done; the results demonstrate the 30 (75%) Pseudomonas aeruginosa. Genetically, study the gene encoding integrin of P. aeruginosa by using the PCR technique. The results indicated that all P. aeruginosa isolates contain the IntClassI gene; PCR amplification revealed that 30 (75%) of P. aeruginosa carried the int1 gene.
Downloads
References
[1] Afolabi OA; Salaudeen AG; Ologe FE; Nwabuisi C & Nwawolo. (2012). CC Pattern of bacterial isolates in
the middle ear discharge of patients with chronic suppurative otitis media in a tertiary hospital in North
central Nigeria. African Health Sciences Vol 12 No 3.
[2] AL-Kraety, I. A. A., & Al-Ammar, M. (2017). RELATION OF CLASS 1 INTEGRON GENE WITH
MULTI-DRUG RESISTANCE SALMONELLA TYPI ISOLATES. Pak. J. Biotechnol. ol, , 53 -
5
[3] Bennett PM. (2008). Plasmid encoded antibiotic resistance: acquisition and transfer of antibiotic resistance
genes in bacteria. Br J Pharmacol.
[4] Chen J, Su Z, Liu Y, Wang S, Dai X, Li Y, et al. (2009). Identification and characterization of class 1
integrons among Pseudomonas aeruginosa isolates from patients in Zhenjiang, China. Int J Infect Dis.
[5] Fonseca EL, Vieira VV, Cipriano R, Vicente AC. (2005). Class 1 integrons in Pseudomonas aeruginosa
isolates from clinical settings in the Amazon region, Brazil. FEMS Immunol Med Microbiol.
[6] Gu B, Tong M, Zhao W, Liu G, Ning M, Pan S, et al. (2007). Prevalence and characterization of class 1
integrons among Pseudomonas aeruginosa and Acinetobacter baumannii isolates from patients in Nanjing,
China. J Clin Microbiol.
[7] Hall Stoodley L, Costerton JW, Stoodley P. (2004). Bacterial biofilms: From the natural environment to
infectious diseases. Nat Rev Microbiol 2(2):95-108.
[8] Hansson K, Sundström L, Pelletier A, Roy PH. (2002).IntI2 integron integrase in Tn7. J Bacteriol.
[9] Khosravi L, Tee Tay S, Vadivelu J. (2011). Analysis of integrons and associated gene cassettes of metallob-lactamase-positive Pseudomonas aeruginosa in Malaysia. J Med Microbiol.
[10] Kumar, H. & Seth, S. (2011). Bacterial and fungal study of 100 cases of chronic suppurative otitis media.
Journal of Clinical and Diagnostic Research, Nov; 5(6): 1224–1227.
[11] MohadesehZarei-Yazdeli, Gilda Eslami, HengamehZandi, Masoumeh Kiani, Kazem Barzegar, Hanieh
Alipanah, SeyedMorteza Mousavi, and Marzieh Shukohifar. (2018). Prevalence of class 1, 2 and 3 integrons
among multidrug-resistant Pseudomonas aeruginosa in Yazd, Iran: Iranian Journal of microbiology: volume
10, number 5.
[12] Mohanna, M. A. B. & Bahannan, A. A. (2016). bacterial profile and antibiogram of otitis media Among
children in Yemen. J Ayub Med Coll Abbottabad; 28(3).
[13] Nikokar I, Tishayar A, Flakiyan Z, Alijani K, Rehana-banisaeed S, Hossinpour M, et al. (2013). Antibiotic resistance and frequency of class 1 integrons among Pseudomonas aeruginosa isolated from burn patients in Guilan, Iran. Iran J Microbiol.
[14] Shrestha BL., Amatya RCM., Shrestha I., Ghosh I. (2011). Microbiological Profile of Chronic Suppurative Otitis Media. Nepalese Journal of ENT Head and Neck Surgery; 2(2): 6–7.
[15] Xu Z, Li L, Shirtliff ME, et al. (2009). Occurrence and characteristics of class 1 and 2 integrons in Pseudomonas aeruginosa isolates from patients in southern China. J Clin Microbiol.
Downloads
Published
Issue
Section
License
Copyright (c) 2020 AUTHOR
This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format for any purpose, even commercially.
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
- The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit , provide a link to the license, and indicate if changes were made . You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Notices:
You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation .
No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.
