Assessment of IL-10 and TGF-β1 among Patients with Celiac Disease
DOI:
https://doi.org/10.61841/10z19073Keywords:
Assessment of IL-10, TGF-β1, Patients with Celiac Disease.Abstract
Celiac disease (CD), also known as "celiac sprue," is a persistent inflammatory condition that involves the small intestine, with a incidence of 1% in the majority of population. This study aimed to investigate the role of some immune parameters such interlukin-10 (IL- 10) and Transforming growth factor-beta1 (TGF-β1) among patients with Celiac Disease. This study was conducted on a total of (75) individual in different sex and age group cases (26 males + 49 females) including (30) patients with Celiac Disease and (15) on a Gluten free diet and
(30) healthy individuals. CD patients were recruited at Imam Sadiq Teaching Hospital, through the duration of the beginning of August 2019 till the end of December 2019. All patients diagnosed with CD by Anti-tTG test. The age range of the study population was from (5-75) years with mean age 32 years. Blood was withdrawn from a vein, for hematology analyzer (total WBCs count, total RBCs count and ESR) the serum was used for immunological tests including IL-10 and TGF-β1 by ELISA technique. The findings revealed that the total WBCs count and ESR were elevated significantly (p< 0.01) in CD patients as (mean= 11.56),(mean= 91) receptively, and CD patients had a significantly lower mean RBC count than healthy Group , 4.39 vs. 4.81 x103 cell/ml, respectively (p=0.01). GFD group had a significantly lower mean RBC count than healthy Group 4.49 vs. 4.81 103 cell/ml, respectively (p=0.01). Celiac Disease patients had a significantly higher mean of IL-10 than the healthy Group, 443.34 vs. 244.87, respectively. (P. value <0.01) GFD group also had a significantly higher mean of IL-10 than the healthy Group, 420.85 vs. 244.87, respectively. as (P. value < 0.01). GFD group had a significantly higher mean of TGF-β1 than CD patients, 1082.09 vs. 387.57, respectively (P. value = 0.01), GFD group also had a significantly higher mean of TGF-β1 than the healthy Group, 1082.09 vs. 72.39, respectively (P. value = 0.01), There was a significant direct (positive) correlation between IL-10 and TGF-b1 (p<0.01).
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[1] Abrams, J. A., Brar, P., Diamond, B., Rotterdam, H., & Green, P. H. (2006). Utility in clinical practice of immunoglobulin a anti-tissue transglutaminase antibody for the diagnosis of celiac disease. Clinical Gastroenterology and Hepatology, 4(6), 726-730.
[2] Al-Rasheid, S. M. A., Al-Sa'adoon, E. R., & Mohammed, M. J. (2010). Evaluation of Procalcitonin Test for Early Diagnosis of Neonatal Sepsis in Tikrit Teaching Hospital. Journal of university of Anbar for Pure science, 4(3), 1-13.
[3] Al-Rawi Kh. M., Entrance to Statistics, 2ed ed., 2000, Babylon, Iraq.
[4] Biancheri, P., Giuffrida, P., Docena, G. H., MacDonald, T. T., Corazza, G. R., & Di Sabatino, A. (2014). The role of transforming growth factor (TGF)-β in modulating the immune response and fibrogenesis in the gut. Cytokine & growth factor reviews, 25(1), 45-55.
[5] Bonetti, T. C. D. S., Salomao, R., Brunialti, M., Braga, D. P. A. F., Borges Jr, E., & Silva, I. D. C. G. D. (2010). Cytokine and hormonal profile in serum samples of patients undergoing controlled ovarian stimulation: interleukin-1β predicts ongoing pregnancy. Human reproduction, 25(8), 2101-2106.
[6] Bray, C., Bell, L. N., Liang, H., Haykal, R., Kaiksow, F., Mazza, J. J., & Yale, S. H. (2016). Erythrocyte sedimentation rate and C-reactive protein measurements and their relevance in clinical medicine. Wmj, 115(6), 317-21.
[7] Forsberg, G., Hernell, O., & Hammarstrom, S. (2007). Concomitant increase of IL-10 and pro- inflammatory cytokines in intraepithelial lymphocyte subsets in celiac disease. International Immunology, 19(8), 993–1001.
[8] Harrison, O. J., & Powrie, F. M. (2013). Regulatory T cells and immune tolerance in the intestine. Cold Spring Harbor perspectives in biology, 5(7), a018341.
[9] Ludvigsson, J. F., Leffler, D. A., & Bai, J. C. (2012). The Oslo definitions for coeliac disease and related terms. Gut, 62(1), 43–52.
[10] Meresse, B., Malamut, G., & Cerf-Bensussan, N. (2012). Celiac disease: an immunological jigsaw.
Immunity, 36(6), 907-919.
[11] Natarajan, S., & Remick, D. G. (2008). The ELISA Standard Save: Calculation of sample concentrations in assays with a failed standard curve. Journal of immunological methods, 336(2), 242-245.
[12] Pagana, K. D., & Pagana, T. J. (2017). Mosby's Manual of Diagnostic and Laboratory Tests-E-Book.
Elsevier Health Sciences.
[13] Rampertab, S. Devi, Nakechand Pooran, Howard W. Sander, and Peter HR Green. "CELIAC DISEASE: ELEVATION OF THE ESR AND ITS RESPONSE TO A GLUTEN-FREE DIET: 191." American Journal
of Gastroenterology 99, no. 10 (2004): S63-S64.
[14] Romero-Adrián, T. B. (2016). Celiac disease: Participation of Cytokines and Other Factors in the Immune Response. Journal of Gastrointestinal Disorders and Liver Function, 1(1), 1–6.
[15] Rubio-Tapia, A., & Murray, J. A. (2010). Celiac disease. Current opinion in gastroenterology, 26(2), 116.
[16] Unsworth, D. J., Lock, F. J., & Harvey, R. F. (1999). Iron-deficiency anaemia in premenopausal women.
The Lancet, 353(9158), 1100.
[17] Veenbergen, S., & Samsom, J. N. (2012). Maintenance of small intestinal and colonic tolerance by IL-10- producing regulatory T cell subsets. Current opinion in immunology, 24(3), 269-276.
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